一株利用生物柴油废水产氢的光合菌的筛选、鉴定  

Screening and identification of a photosynthetic bacterial strain for biohydrogen production from biodiesel wastewater

作　　者：宗文明[1,2] 于瑞嵩[2] 樊美珍[1] 周志华[2] 

机构地区：[1]安徽农业大学微生物防治省重点实验室,安徽合肥230036 [2]中国科学院上海生命科学研究院,植物生理生态研究所,合成生物学重点实验室,上海200032

出　　处：《中国环境科学》2009年第10期1076-1081,共6页China Environmental Science

基　　金：国家“863”项目(2006AA05Z105);上海市国际科技合作基金(063107032)

摘　　要：从37株光合细菌中筛选出1株能够利用甘油做碳源进行有效产氢的菌株(DB803).根据该菌株的形态、生理生化特征、16SrDNA序列和ERIC-PCR结果分析,初步鉴定该菌株为类球红细菌(Rhodobacter sphaeroides).研究了该菌株在30℃,4000lx光照厌氧条件下利用不同浓度的生物柴油废水产氢的能力,当培养基起始COD浓度为11.5g/L时,其在对数生长期平均产氢速度为38mL/(L·h),同时,废水COD去除率达91.2%.A strain （designated as DB803） producing hydrogen efficiently with glycerol as the carbon source was screened from 37 photosynthetic bacterial strains. This strain was identified as Rhodobacter sphaeroides based on its phenotype, physiological and biochemical characteristics, the sequence of its 16S rRNA gene and fingerprinting of ERIC-PCR. Its hydrogen yields from biodiesel wastewater at different dilution times were investigated under the anaerobic condition of 30℃, 4000lx of illumination. The average hydrogen-producing rate of 38mL/（L·h） was observed at log phase when the initial COD value of wastewater was diluted to 11.5g/L. Meanwhile, the COD decreased greatly with a removal efficiency of 91.2% at batch test.

关 键 词：生物制氢 光合细菌 甘油 生物柴油废水 

分 类 号：X172[环境科学与工程—环境科学]