微波合成葡寡糖的体外抗氧化作用的研究  

Study on Anti-oxidative Capacity of Glucooligosaccharides Synthesized by Microwave-assistance

作　　者：刘云[1] 宋玉蓉[1] 乐国伟[2,1] 施用晖[2,1] 

机构地区：[1]江南大学食品学院营养与食品安全研究所,江苏无锡214122 [2]食品科学与技术国家重点实验室,江苏无锡214122

出　　处：《食品与发酵工业》2009年第10期36-39,共4页Food and Fermentation Industries

基　　金：国家自然基金资助(30571347);科技部"十一五"支撑计划项目(2006BAD27B06)

摘　　要：对微波合成葡寡糖(GOS)进行了高效液相色谱(HPLC)、红外光谱(IR)、表面疏水性(S0)分析,研究了3种不同聚合度葡寡糖(LGOS、MGOS、HGOS)对.OH、DPPH、ABTS+.3种自由基的清除作用。结果表明:葡寡糖对.OH自由基、DPPH自由基清除作用随着聚合度、浓度及疏水性的增加而增强,HGOS的清除能力最强,IC50分别为66mg/mL和44mg/mL;对水溶性自由基ABTS+.清除能力随着葡寡糖聚合度、浓度及疏水性的增加呈降低趋势,LGOS、MGOS对ABTS+.自由基的IC50分别为74.7mg/mL和78mg/mL,HGOS的最高清除率达到48.3%。The article analyzed the glucooligosaccharides synthesized with microwave-assistance by HPLC and IR and studied its surface hydrophobicity （ S0） . We also studied the scavenging activity of glucooligosaccharides with different degrees of polymerization to · OH radical, DPPH radical and ABTS^＋· radical. The results showed that the scavenging activity of glucooligosaccharides to · OH radical and DPPH radical increased with the degree of polymerization, concentration and surface hydrophobicity increase. The scavenging activity of HGOS was the best and its IC50 was about 66 mg/mL and 44 mg/mL. The scavenging activity to Water-soluble ABTS^＋ · radical reduced with the degree of polymerization, concentration and increased surface hydrophobicity. The IC50 of LGOS and MGOS were 74.7 mg/mL and 78 mg/mL. The best scavenging activity of HGOS was 48.3%.

关 键 词：微波合成 葡寡糖 抗氧化 自由基 

分 类 号：R151[医药卫生—营养与食品卫生学]