午间花棒(Hedysarum scoparium)不同器官对水分胁迫的抗氧化保护  

The Antioxidative Protection Strategies of Different Organs in Hedysarum scoparium under Water Stress at Midday

作　　者：龚春梅[1] 白娟[1] 王根轩[2] 曹翠玲[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨陵712100 [2]浙江大学生命科学学院农业生态工程研究所,浙江杭州310058

出　　处：《西北林学院学报》2009年第6期15-19,共5页Journal of Northwest Forestry University

基　　金：西北农林科技大学人才基金(01140411);国家自然科学基金(90102015)

摘　　要：通过测定不同地下水埋深(1.4、2.43、.4 m)条件下花棒(Hedysarum scoparium)抗氧化酶类(SOD、POD和CAT)活性和抗氧化剂类黄酮含量,分析花棒叶和叶轴抗氧化系统在水分胁迫加剧情况下对午间高温干旱的保护作用。结果表明:随土壤水分胁迫加剧,午间花棒叶SOD活性、叶轴SOD活性和叶轴POD活性逐渐增加,叶POD活性、叶CAT活性和叶轴CAT活性先增后减,叶与叶轴的SOD和POD活性比值逐渐减小,花棒叶和叶轴类黄酮的积累增加;且在每一地下水位深度,花棒叶抗氧化酶活性高于叶轴,花棒叶类黄酮相对含量高于叶轴,叶与叶轴类黄酮的比值相近。由此表明,随水分胁迫加剧,花棒叶对午间高温干旱的防御能力均强于叶轴;抗氧化酶活性的增加是花棒应对水分胁迫加剧的策略之一,并且随土壤水分胁迫加剧,叶轴的抗氧化能力逐渐提高;花棒应对水分胁迫加剧的另一策略是叶与叶轴类黄酮相对含量增加,并且增加过程中二者比值保持稳定。The aim of this study was to test the protective role of superoxide dismutase （SOD）, guaiacol peroxidase （POD）, catalase （CAT） and flavonoids against oxidative damage in the different soil water stress conditions at midday with high temperature and drought in Hedysarurn scoparium. The results showed that the SOD activities of leaves and rachis, the POD activities of rachis increased gradually, and the POD activities of leaves, the CAT activities of leaves and rachis increased firstly and decreased later, and the ratio of SOD, and POD in leaves/rachis declined, and the accumulation of flavonoids increased in leaves and rachis with increasing water stress in midday. In addition, activities of antioxidases were higher in leaves than thonse in rachis, and flavonoids were higher in leaves than those in rachis, and the ratio of flavonoids in leaves/rachis was in a stable level at each depths of ground water. Conclusions were as follows： （1} the capacity of defence to high temperature and drought was stronger in leaves than that in rachis; （2） increasing of antioxidases is a strategy to resist water stress,meanwhile anti oxidative capacity in rachis enhanced gradiently with increasing soil water stress; （3） to increase relative content of flavonoids is another strategy to resist increasing soil water stress, and ratio of flavonoids in leaves/rachis was stable.

关 键 词：花棒 高温 干旱 抗氧化酶 类黄酮 

分 类 号：S793.901[农业科学—林木遗传育种]