Photofrin-Diomed 630-PDT对人永生化食管上皮细胞系SHEE及其癌变细胞系SHEEC的抑制研究  

The Inhibiting Effect of Photofrin-Diomed 630-PDT Photodynamic Therapy on Human Immortalization Eesophageal Epithelial Cell Line SHEE and Its Malignant Transformation Cell Line SHEEC

作　　者：高社干[1,2] 王立东[1] 冯笑山[1,2] 曲智锋[2] 单探幽[2] 谢萱虎[2] 

机构地区：[1]郑州大学基础医学院癌症研究室,河南省食管癌重点开放实验室,郑州450052 [2]河南科技大学第一附属医院肿瘤科,肿瘤研究所,河南洛阳471003

出　　处：《中国医科大学学报》2009年第10期730-733,共4页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30670956);国家高技术研究发展计划(863计划)项目(2006AA02A403)

摘　　要：目的探讨肿瘤细胞对光动力的敏感度,同时为体外细胞培养的光动力实验研究选择最佳光剂量和最佳光密度提供实验依据。方法将人永生化食管上皮细胞系SHEE及其癌变细胞系SHEEC分别随机分成21组,接种24h贴壁后,在30μg/ml的光敏剂photofrin-Ⅱ溶液中孵育150min后,在15min内应用Diomed-630型激光仪给予25mW/cm2、50mW/cm2、100mW/cm23个功率密度分别照射10,20,30,50,100,150,200s,然后继续培养24h,应用缩胆囊胆素八肽(CCK-8)法检测两个细胞系的存活率。结果在photofrin-Ⅱ30μg/ml浓度条件下,相同功率密度的光动力疗法(PDT)对细胞系SHEE和SHEEC的抑制率无显著性差异;在photofrin-Ⅱ30μg/ml浓度条件下,随着光剂量的增加PDT对细胞系SHEE和SHEEC的抑制率呈增加趋势,达到2.5～3.0J/cm2后进入平台期。结论人永生化食管上皮细胞系SHEE及其癌变细胞系SHEEC对光动力的敏感度无显著性差异,提示肿瘤光动力治疗的靶向作用可能与肿瘤细胞对激光的敏感性无关。ObjeCtive To investigate the sensitivity, of tumor cells to photodynamic therapy（PDT）, and to select the optimal photo-dose and photo density for PDT to the cultured cells. Methods Cells of SHEE and SHEEC cell lines were divided into 21 groups randomly,after 24 h inoeulation,the cells accreted on paries of culture capsule completely,we replaced M199 complete culture solution with 30 μg/nd Photofrin- Ⅱ solution 100 and then replacd with M199 complete culture solution without Photofrin- Ⅱ ＇after 150min of incubation in Photofrin- Ⅱ. Within 15 min ,we dealed the cells with PDT using Photofrin-Diomed 630 under three different power densities：25 mW/cm2, 50 mW/cm2 and 100 mW/cm2 for 10 s,20 s,30 s,50 s, 100 s, 150 s and 200 s respectively,and then continue for 24 h culture.. We examined the inhibiting effect on cell line SHEE and SHEEC under PDT by the method of CCK-8. Results There was no signifieant difference in the inhibition ratio with same power density of PDT between cell line SHEE and SHEEC under the concentration of 30 μg/ml Photofrin- Ⅱ. However,the inhibition ratio increased with the raising of photo-dose of PDT, but there was a platform stage after the 2.5-3.0 J/em2 photo-dose. Conclusion The difference for photodynamic sensitivity between human immortalization esophageal epithelial cell hne SHEE and the malignant transformation cell line SHEEC is not significant. The targeting of PDT to malignant tumor cell may not be involved in the photosensitivity for tumor cell.

关 键 词：细胞系SHEE 细胞系SHEEC 光动力疗法 photofrin-Ⅱ 

分 类 号：R734.2[医药卫生—肿瘤] R780.2[医药卫生—临床医学]