小鼠钟状期牙胚中Frizzled蛋白的表达  

Expression of Frizzled Protein in Mouse Tooth Germ at Bell Stage

作　　者：高洁[1] 叶新萍 高妍[1] 刘飞[1] 

机构地区：[1]中国医科大学附属口腔医院儿童牙科,沈阳110002 [2]辽河油田第二医院口腔科,辽宁盘锦124010

出　　处：《中国医科大学学报》2009年第10期755-757,共3页Journal of China Medical University

基　　金：辽宁省自然科学基金资助项目(20052086)

摘　　要：目的观察牙胚发育钟状期时Frizzled蛋白的表达情况,探讨Wnt/Frizzled信号分子的作用及机制。方法取胚胎17d和19d的胎鼠,另取生后2d的幼鼠,分别制作下颌第1磨牙的切片,运用免疫荧光技术显示Frizzled蛋白的阳性分布部位,并在荧光显微镜下观察。结果胚胎17d时Frizzled蛋白在成釉器的内、外釉上皮有弱阳性分布,胚胎19d时在前成釉细胞和前成牙本质细胞的顶端有Frizzled蛋白的阳性表达,生后2d的标本上成釉细胞和成牙本质细胞的顶端Frizzled蛋白呈强阳性表达。结论Wnt/Frizzled信号分子参与了成釉细胞和成牙本质细胞的分化过程的调节。Objective To observe the expression of Frizzled protein in mouse tooth germ at bell stage and explore the possible function of Wnt/Frizzled signal molecules. Methods Mouse embryos at 17 and 19 days of gestation （E17 and E19） as well as mice postnatal day 2 （PN2） were employed in our study. The frozen sections of the first lower molar were made and indirect immunofluorescence technique was carried out on the sections. The location of Frizzled protein was observed under the fluorescence microscope. Resulls Frizzled protein was expressed weakly in the inner- and outer- dental epithelium at El7. At E19,the positive staining was found to distribute at the summit of both pre-ameloblasts and pre-odontoblasts. At P2, Frizzled protein was expressed strongly at the summit of ameloblasts and odontoblasts. Conclusion Wnt/Frizzled signal molecules may be involved in the differentiation of ameloblasts and odontoblasts.

关 键 词：牙胚 钟状期 Frizzled蛋白 成釉细胞 成牙本质细胞 

分 类 号：R780.2[医药卫生—口腔医学]