肺癌患者外周血BJ-TSA-9,LUNX和CK19 mRNA的检测及其临床意义  

Detection of BJ-TSA-9,LUNX and CK19 mRNA in Peripheral Blood and Its Diagnostic Value in Lung Cancer

作　　者：刘鹏辉[1] 廖国清[1] 李月敏[1] 尹艳慧[2] 王红梅[1] 曲怡梅[1] 

机构地区：[1]解放军总医院第二附属医院肿瘤科,北京100091 [2]北京大学医学部免疫学系T细胞研究室,北京100083

出　　处：《中国医科大学学报》2009年第10期786-789,共4页Journal of China Medical University

摘　　要：目的观察肺癌患者外周血循环中BJ-TSA-9,LUNX和CK19mRNA的表达情况,并探讨BJ-TSA-9,LUNX和CK19mRNA联合检测的临床意义。方法采用巢式逆转录聚合酶链反应(Nested-RT-PCR)技术检测84例肺癌患者、32例肺良性病变患者和20例健康人。结果肺癌患者外周血中BJ-TSA-9,LUNX和CK19mRNA阳性率分别为59.5%,40.4%和22.6%。32例良性病变者外周血中BJ-TSA-9,LUNX和CK19mRNA阳性率分别为9.3%,12.5%和6.3%。20例健康志愿者外周血中无BJ-TSA-9,LUNX和CK19mRNA表达。Ⅳ期肺癌患者BJ-TSA-9,LUNX和CK19mRNA阳性率明显高于Ⅱ期和Ⅲ期(P<0.05)。结论BJ-TSA-9,LUNX和CK19mRNA均可作为RT-PCR法检测肺癌患者外周血微转移的肿瘤标志物。BJ-TSA-9mRNA在有效率上优于LUNXmRNA和CK19mRNA,肺癌患者外周血中检测出BJ-TSA-9mRNA提示可能有血循环转移。Objective To investigate the detection of BJ-TSA-9 mRNA,LUNX and CK19 mRNA in peripheral blood cells of lung cancer patients and evaluate its diagnostic value. Methods The expression of BJ-TSA-9,LUNX and CKI9 mRNA in peripheral blood cells was detected from 84 lung cancer patients, 32 benign lung lesions and 20 healthy volunteers by nested reverse transcription polymerase chain re- action （Nested-RT-PCR）. Results The positive detection rates of BJ-TSA-9,LUNX and CK19 mRNA in the peripheral blood of the pa- tients with lung cancer were 59.5% ,40.4%and 22.6% respectively. In the 32 peripheral blood samples of the patients with benign lung lesions,the positive detection rate of BJ-TSA-9,LUNX and CK19 mRNA were 9.3%,12.5% and 6.3% respectively. No expression of BJ-TSA- 9, LUNX and CK19 was detected in the samples of the healthy volunteers. The expression level of BJ-TSA-9, LUNX and CK19 mRNA in the peripheral blood of lung cancer stage IVpatients was significantly higher than those of stages Ⅱ and Ⅲ （P 〈 0.05）. Conclusion RT- PCR amplification of BJ-TSA-9,LUNX and CK19 mRNA are an efficient way to detect early haematogenous dissemination of cancer cells for lung cancer patients. The detection of BJ-TSA-9 expression is sensitive and specific for lung cancer,and it is superior to both LUNX and CKI9 mRN,A. The expression of BJ-TSA-9 mRNA in peripheral blood might predict the hematogenous metastatic spreading of lung cancer cells.

关 键 词：肺癌 多聚酶链反应 外周血 

分 类 号：R734.2[医药卫生—肿瘤]