苯乙酸对大鼠胶质瘤C6细胞凋亡的影响  

Effect of phenylacetate on apoptosis in rat C6 glioma cells in vivo

作　　者：杨朝华[1] 游潮[1] 黄少伟[2] 王光绿[2] 李齐广[2] 韩方华[2] 

机构地区：[1]四川大学华西医院神经外科,四川成都610041 [2]北海市人民医院神经外科,广西北海536000

出　　处：《西部医学》2009年第11期1841-1843,共3页Medical Journal of West China

基　　金：广西科学基金资助项目(No.桂科青0640078)

摘　　要：目的观察苯乙酸(Phenylacetate,PA)对大鼠胶质瘤C6细胞凋亡的影响。方法建立胶质瘤大鼠动物模型20个,其中治疗组10个给予腹腔注射PA治疗,对照组10个给予生理盐水注射,分别观察至濒死期获取标本,HE染色,TUNEL检测细胞凋亡。结果治疗组大鼠平均生存时间(31.3±3.1)d比对照组平均生存时间(23.4±3.8)d增加,TUNEL检测治疗组细胞凋亡率(27.1±2.6)%比对照组凋亡率(1.8±0.3)%增加。结论在体内PA能够抑制肿瘤生长,诱导胶质瘤C6细胞凋亡。Objective To observe the effect of phenylacetate （PA） on apoptosis in rat C6 glioma cells in vivo. Methods C6 cells were transplanted into the brain of Wistar rats and 20 glioma models were established. 10 rats in treatment group were administered PA by peritoneal injection, while 10 rats in control group were administered 0. 9% sodium chloride solution. The rats were observed and specimens were collected just before dying. The pathologic changes were observed by HE staining. Apoptosis was detected by TUNEL assay. Results 20 glioma models were established successfully. The survival time of treatment group （31.3±3.1） d was longer than that of control group （23.4 ± 3.8）d. TUNEL assay showed that the rate of apoptosis in treatment group （27.1±2.6） % was higher than that of control group （1.8±0.3） %. Conclusions PA can inhibit the growth of glioma and induce apoptosis in rat C6 glioma cells in vivo.

关 键 词：苯乙酸 胶质瘤 凋亡 

分 类 号：R730[医药卫生—肿瘤]