弥漫大B型非霍奇金淋巴瘤患者外周血TCR Vβ亚家族T细胞分布和克隆性增生特点  

Feature of distribution and clonal expansion of TCR Vβ subfamily in T cells from peripheral blood in patients with DLBL

作　　者：叶静梅[1] 谭获[1] 李扬秋[2] 陈少华[2] 岑东芝[2] 陈思[2] 吴秀丽[2] 

机构地区：[1]广州医学院第一附属医院肿瘤血液中心,广州510230 [2]暨南大学医学院血液病研究所

出　　处：《临床血液学杂志》2009年第6期594-596,共3页Journal of Clinical Hematology

摘　　要：目的:了解弥漫大B淋巴瘤(DLBL)患者外周血T细胞的TCRVβ基因谱系及克隆性增殖情况。方法:利用RT-PCR方法扩增6例DLBL患者外周血单个核细胞中24个TCRVβ基因的互补决定区3(CDR3),PCR产物进一步经荧光标记和基因扫描分析CDR3长度而确定T细胞的克隆性。结果:6例DLBL患者分别表达9～20个TCRVβ亚家族。患者均存在1个或多个Vβ亚家族的克隆增殖T细胞,增殖形式包括寡克隆、寡克隆增殖趋势及双克隆。Vβ13和Vβ15亚家族出现克隆增殖性T细胞的频率较高(66.7%)。结论:DLBL患者外周血TCRVβ亚家族T细胞的分布具有特点,表现为TCRVβ亚家族T细胞呈限制性选用和克隆性增殖,这可能是淋巴瘤患者机体对淋巴瘤相关抗原产生的特异性免疫反应。Objective：To investigate the situation of TCR Vβ genealogy and clonal expansion in T cells from peripheral blood of patients with diffuse large B-cell Lymphoma（DLBL）.Method：The complementarity determining region 3（CDR3） of 24 TCR Vβ subfamily genes in peripheral blood mononuclear cell from 6 cases with DLBL was amplified using RT-PCR.The products were further labelled by fluorescein and then analyzed the CDR3 size by genes-scan technique,thus determining clonality of T cells.Result：9-20 Vβ subfamilies were identified in 6 DLBL cases.Clonal expansive T cells in one or more Vβ subfamilies were found in all 6 patients,including oligoclonal/oligoclonal trend and bioclonal patterns.The higher frequency of clonal expansive T cells was predominately in Vβ13 and Vβ15 subfamilies（66.7%）.Conclusion：There is some special feature in the distribution of TCR Vβ subfamily T cells in DLBL.The restricted usage and clonal expansion of TCR Vβ subfamily T cells could be found on the patients with DLBL.This feature may be related to the host immune response to DLBL associated antigen.

关 键 词：弥漫大B细胞性淋巴瘤 T细胞受体VΒ基因 T细胞克隆性 基因扫描 

分 类 号：R733.4[医药卫生—肿瘤]