TNF-α可诱导乳腺癌细胞凋亡  

TNF-α Induced Apoptosis of Breast Cancer Cells

作　　者：温媛媛[1] 杨志强[1] 宋敏[1] 李柏林[2] 陈晓玲[1] 姚晓慧[1] 

机构地区：[1]中国医科大学基础医学院病理学教研室,辽宁沈阳110001 [2]中国医科大学化学教研室,辽宁沈阳110001

出　　处：《中国血液流变学杂志》2009年第3期349-351,374,共4页Chinese Journal of Hemorheology

基　　金：辽宁省教育厅资助项目（05L514）

摘　　要：目的研究TNF-α对乳腺癌的影响。方法采用RT-PCR和WesternBlotting分析30例乳腺浸润性导管癌及癌旁正常乳腺组织，乳腺正常上皮细胞系及乳腺癌细胞系中TNF-α的表达情况；采用流式细胞术观察TNF-α对乳腺癌细胞凋亡的影响。结果RT-PCR和Western Blotting结果碌示，TNF-αmRNA和蛋白在乳腺癌组织中表达都明显低于配对的癌旁正常组织（P〈0．05），在乳腺上皮细胞系中表达均高于乳腺癌三利一细胞系，流式细胞术检测结果显示与未处理组桐比．经TNF-α处理的MDA-MB-435S（16．7±0．31）和MCF7（18．6±0．42）细胞的凋亡率明显增加，差异均有统计学意义（P〈0．05）。结论TNF-α可促进乳腺癌细胞的凋亡，TNF-α可为乳腺癌的治疗提供新靶点。Objective To investigate the effection of TNF-α on the breast cancer.Method The expression of TNF-α mRNA and protein was examined in 30 frozen tissues, breast epithelium cell line（MCF-10A） and breast cancer cell lines（MCF7,MDA-MB-231,MDA-MB-435S） by RT-PCR and Western Blotting. The influence of TNF-α on breast cancer cells was detected with Flow Cytometry. Result RT-PCR and Western Blotting analysis showed there was a significant decrease in expression of TNF-α mRNA and protein in breast cancer tissues compared with the adjacent normal breast tissues （P〈0.05）,and there was a marked increase in expression of TNF-α mRNA and protein in breast epithelium cell line （MCF-10A） compared with breast cancer cell Iines（MCF7,MDA- MB-231,MDA-MB-435S）. Flow Cytometry analysis showed there was an distinguished increase in apoptosis rate ofMDA-MB-435S（16.7±0.31）and MCF7（18.6±0.42）treated with TNF-α than the untreated （2.3 ± 0.14, 1.9 ± 0.22 respectively）.Conclusion The results strongly imply that TNF-α play an important role in apoptosis of breast cancer cells, and TNF-α could be a potential target for the treatment of the patients with breast cancer.

关 键 词：乳腺癌 TNF-Α 凋亡 

分 类 号：R730.2[医药卫生—肿瘤]