免疫组织化学法诊断恶性淋巴瘤的研究——(Ⅰ,冰冻切片)  

An Immunohistochemical Study in Diagnosis of Malignant Lymphoma Ⅰ—on Frozen Sections

作　　者：祁陈凤 高见刚[1] 尾岛昭次 冼美生[2] 

机构地区：[1]日本岐阜大学医学部病理学教研室 [2]河北医学院病理学教研室

出　　处：《临床与实验病理学杂志》1990年第4期253-256,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：应用ABC免疫组织化学方法标记30多种单克隆抗体,对200例临床诊断为恶性淋巴瘤的病人用冰冻切片进行了研究。观察结果提示真性恶性淋巴瘤175例,各种抗淋巴细胞单克隆抗体反应证明肿瘤性病变均为单克隆性增殖,而炎症反应性病变则为多克隆性增殖。单克隆抗体CD_2、CD_3、CD_5,CD_7对T细胞恶性淋巴瘤呈阳性反应;CD_(10)、CD_(10)、CD_(20)、CD_(22)、L-26对B细胞恶性淋巴瘤呈阳性反应:CD_4、CD_8对判断T细胞恶性淋巴瘤T_(h/i)、T_(c/s);亚群非常有效。Ki-1、Leu-M_1,对诊断何杰金氏病相当有效。One-hundred cases which had been diagnosed clinically as malignant lymphoma were studied immunohistochemically by using ABC method. Various anti-leucocyte monoclonal antibodies and polyclonal anti-sera against immunoglobulin or other proteins that exist in paraffin sections were used. Of these 100 cases, 81 cases were diagnosed as non-Hodgkin's lymphoma, and 9 cases as Hodgkin's disease. The other 10 cases were consisted of histiocytic necrotizing lympha- denitis, metastasis of poorly differentiated carcinoma, and non-speci fic reactive lymphadenitis. Among those anti-leucocyte monoclonal antibodies, MT-1 and UCHL-1 were positive for T-cell lymphomas, while MB-1, MB-2, MB-3, LN-2, L-26 were reactive with B-cell lymphomas. Leu-M was only positive for Hodgkin's disease. The results confirmed that the use of immunohistochemistry is valuable for the diagnosis in malignant lymphoma.

关 键 词：淋巴癌 免疫组织化学 冰冻切片 

分 类 号：R733.404[医药卫生—肿瘤]