免疫组织化学法诊断恶性淋巴瘤的研究——(Ⅱ,石蜡切片)  

An Immunohistochemical Study in Diagnosis of Malignant: Lymphoma——Ⅱ—On Routine Paraffin Section

作　　者：祁陈凤 高见刚[1] 尾岛昭次 冼美生[2] 

机构地区：[1]日本岐阜大学医学部病理学教研室 [2]河北医学院病理学教研室

出　　处：《临床与实验病理学杂志》1990年第4期257-265,共9页Chinese Journal of Clinical and Experimental Pathology

摘　　要：应用ABC免疫组化方法标记10多种单克隆抗体,用常规石蜡切片对100例临床诊断为恶性淋巴瘤的病例进行了研究。结果证明,在石蜡切片中能对恶性淋巴瘤发生阳性反应。否认了以往认为抗淋巴细胞各种单克隆抗体仅能用于冰冻切片新鲜淋巴组织的论点,检出淋巴瘤81例(B细胞性71例、T细胞性10例),何杰金氏病9例。单克隆抗体MT-1、CUHL-1、对T细胞性淋巴瘤呈阳性反应,MB-1、MB-2、LN-2、LN-3、L-26对B细胞性淋巴瘤呈阳性反应。Leu-M_1对何杰金氏病的诊断可起相当大的作用。One-hundred cases which had been diagnosed clinically as malignant lymphoma were studied immunohistochemically by using ABC method. Various anti-leucocyte monoclonal antibodies and polyclonal anti-sera against immunoglobulin or other proteins that exist in paraffin sections were used. Of these 100 cases, 81 cases were diagnosed as non-Hodgkin's lymphoma, and 9 cases as Hodgkin's disease. The other 10 cases were consisted of histiocytic necrotizing lympha- denitis, metastasis of poorly differentiated carcinoma, and non-speci fic reactive lymphadenitis. Among those anti-leucocyte monoclonal antibodies, MT-1 and UCHL-1 were positive for T-cell lymphomas, while MB-1, MB-2, MB-3, LN-2, L-26 were reactive with B-cell lymphomas. Leu-M was only positive for Hodgkin's disease. The results confirmed that the use of immunohistochemistry is valuable for the diagnosis in malignant lymphoma.

关 键 词：淋巴癌 免疫组织化学 石蜡切片 

分 类 号：R733.404[医药卫生—肿瘤]