香菇多糖对鸡球虫3-1E重组蛋白的免疫增强作用  

Adjuvant effect of lentinan on the immune responses to recombinant 3-1E protein against coccidiosis

作　　者：施伟领[1,2] 徐慧[1] 叶秀娟[3] 杜爱芳[1] 

机构地区：[1]浙江大学动物科学学院农业部动物疫病病原学与免疫控制重点开放实验室,浙江杭州310029 [2]浙江大飞龙动物保健品有限公司,浙江金华321017 [3]金华职业技术学院,浙江金华321007

出　　处：《中国兽医学报》2009年第11期1444-1449,共6页Chinese Journal of Veterinary Science

基　　金：金华市科技局项目(2007-2-001)

摘　　要：将150只海蓝雏鸡分为5组,每组30只,3日龄首免,2周后加强免疫1次,二免后2周用鸡柔嫩艾美尔球虫攻击。通过测定免疫后鸡只的淋巴细胞转化水平,抗体水平I、L-1诱生活性,比较分析不同剂量的香菇多糖对3-1E重组蛋白免疫原性的增强作用;通过测定攻虫后鸡只存活率、相对增重率、卵囊值和抗球虫指数的变化,分析香菇多糖对3-1E重组融合蛋白免疫保护效果的影响。结果表明,香菇多糖能够显著增强淋巴细胞转化水平(P<0.05)和血清抗体水平(P<0.05),但不能明显提高IL-1的诱生活性(P>0.05);而且能够显著增强3-1E重组蛋白的免疫保护效果。表明香菇多糖对3-1E重组蛋白具有较好的免疫增强作用。One hundred fifty chickens were allocated to five groups（30/group）. Recombinant 3-1E protein and lentinan solution were administrated by intramuscular on d 3, respectively; then boosted after two weeks; and challenged with Eimeria tenella after four weeks. The immunogenic differences were analyzed via the successive investi-gation on the level of lymphocyte transformation, serum antibody titer and IL-1 induced activity, as well as the immu- noprotection via the survey of the survival, the relative weight gain,oocyst output and anti-coccidiosis index. Lentinan could significantly enhance the proliferation responses of blood lymphocytes（P〈0.05）and the level of serum antibody titer（P〈0.05）. Meanwhile,it could greatly lift the protection induced by recombinant 3-1E protein against E. tenella. The results indicated a strong enhancement of immune responses to recombinant 3-1E protein by co-adminis- tration with lentinan.

关 键 词：香菇多糖 佐剂 鸡球虫 免疫 

分 类 号：S859.7[农业科学—临床兽医学]