Ghrelin对水牛体外受精和孤雌激活胚胎体外发育的影响  

Effects of Ghrelin on in vitro development of buffalo in vitro fertilized and parthenogenetic embryos

作　　者：谢体三[1] 农微[1] 张新民[1] 覃琦丽[1] 黄雅琼[1] 石德顺[1] 

机构地区：[1]广西大学动物繁殖研究所,广西南宁530004

出　　处：《中国兽医学报》2009年第11期1478-1480,共3页Chinese Journal of Veterinary Science

基　　金：国家"863"计划资助项目(2002AA06651)

摘　　要：本研究的目的是探讨Ghrelin对水牛体外受精和孤雌激活胚胎体外发育的影响。体外成熟的水牛卵母细胞经体外受精或离子霉素孤雌激活后,分别在含0,0.5,5,50和500μg/L Ghrelin的培养液中进行体外培养,观察各组胚胎的卵裂率和囊胚率。结果显示,在培养液中添加不同浓度的Ghrelin对体外受精和孤雌激活胚胎的卵裂率均无显著影响(P>0.05),但添加500μg/L的Ghrelin显著提高体外受精胚胎的囊胚发育率(33.5%vs 13.7%,P<0.05),50μg/L或500μg/L的Ghrelin均显著提高孤雌激活胚胎的囊胚发育率(32.4%和34.6%vs 14.5%,P<0.05)。结果表明,培养液中添加Ghrelin对胚胎的早期卵裂没有影响,但可促进水牛体外受精和孤雌激活胚胎囊胚的形成。The objective of this study was to investigate the effect of addition of Ghrelin to in vitro culture medium on preimplantation development of buffalo in vitro fertilized and parthenogenetic embryos. The final concentration of Ghrelin,0,0.5,5,50,500μg/L, respectively, were added in the culture medium during the early embryo development stage. The results showed that,no prominent different was found between the Ghrelin treated group and the control in parthenogenetic embryos or in vitro fertilized embryos. In in vitro fertilized group, the blastocyst rate was remarkably（22.17% vs 6.67% ,P〈0.05） increased when 500μg/L of Ghrelin was added in vitro culture medium compared with the control, while there was no significant difference among the other Ghrelin treated group. In parthenogenetic embryos, the blastocyst rate was significantly（32% vs 14.47%, P〈0.05）enhanced when 50μg/L of Ghrelin was added to culture medium compared with the control. The results suggest that Ghrelin can enhance blastocyst formation of buffalo in vitro fertilized and parthenogenetic embryos while has no effect on the early cleavage.

关 键 词：GHRELIN 水牛 体外受精 孤雌激活 胚胎培养 

分 类 号：S823.83[农业科学—畜牧学]