虾青素对过氧化氢所致质膜氧化损伤的保护作用  

Protective effects of astaxanthin on the oxidative damage of plasma membrane induced by hydrogen peroxide

作　　者：曹秀明[1,2] 王宗利[1,2] 杨贵群[1,2] 

机构地区：[1]国家教育部抗肿瘤天然药物工程研究中心,黑龙江哈尔滨150076 [2]哈尔滨商业大学生命科学与环境科学研究中心,博士后工作站,黑龙江哈尔滨150076

出　　处：《中国海洋药物》2009年第5期44-50,共7页Chinese Journal of Marine Drugs

摘　　要：目的探讨虾青素对H_2O_2所致质膜氧化损伤的保护作用。方法采用H_2O_2导致红细胞膜氧化损伤,以细胞形态学、细胞溶血率、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、膜封闭能力及流动性为指标,观察一定浓度的虾青素(1.0×10^(-7)、1.0×10^(-6)、1.0×10^(-5)mol·L^(-1))对H_2O_2所致质膜氧化损伤的保护作用。结果H_2O_2能导致红细胞溶血率和MDA含量增加、SOD活性、膜封闭能力及膜流动性下降。而经过一定浓度的虾青素预处理后,红细胞溶血率和MDA含量明显降低、SOD活性、膜封闭能力及膜流动性显著增强。结论虾青素对H_2O_2引起的质膜氧化损伤具有明显的保护作用,其机制可能与其抗氧化作用有关。Objective To test the hypothesis that astaxanthin may protect the plasma membrane from oxidative damage induced by hydrogen peroxide（H2O2 ）. Methods Using H2O2- induced red blood cells damage as the model of oxidative damage of the plasma membrane, the protective effect ofastaxanthin （1.0×10^-7、1.0×10^-6、1.0×10^-5mol·L^-1） onoxidated erythrocyte ghost was observed by the measurement of the haemolysis of the erythrocyte, the morphology of the erythrocyte, the content of malonidial aldehyde（MDA）, the enzymatic activity of the superoxide dismutase （SOD）, the membrane fluidity and the blocking degree. Results The rate of hematolysis and erythrocyte MDA content were increased significantly and SOD activity, membrane fluidity and blocking degree of the erythrocyte were decreased owing to the use of H2O2. When the astaxanthin was pretreated, the rate of hematolysis and MDA content of erythrocyte were decelerated obviously and the erythrocyte SOD activity, erythrocyte membrane fluidity and blocking degree were accelerated obviously. Conclusion Astaxanthin showed protective effect on the oxidative damage of plasma membrane induced by H2O2, and the possible mechanism was relevant with its antioxidation.

关 键 词：虾青素 脂质过氧化 膜封闭度 膜流动性 

分 类 号：R96[医药卫生—药理学]