BSTR酶反应器中布洛芬的酶促拆分及反应动力学研究  

Resolution of Racemic Ibuprofen in BSTR Enzymatic Reactor and Kinetic Study of the Reaction

作　　者：唐良华[1] 苏敏[1] 陈梦虹[1] 吕博彦[1] 蔡志雄[1] 

机构地区：[1]福建师范大学生命科学学院,福建福州350108

出　　处：《福建师范大学学报（自然科学版）》2009年第6期71-76,共6页Journal of Fujian Normal University：Natural Science Edition

基　　金：福建省自然科学基金资助项目(2007J0062;2009J01125);福建师范大学优秀青年骨干教师培养基金资助项目(2008100242)

摘　　要：为了提高固定化扩展青霉TS414脂肪酶(PEL)对外消旋布洛芬的拆分效率,利用均匀设计的实验方法,在酶反应器中考察和优化了布洛芬、固定化脂肪酶、1-丙醇等几个参数对拆分反应的影响.优化后的实验结果表明:当反应体系中布洛芬为200 mg,固定化酶的加量为6 g,1-丙醇为0.383 mL,反应达到平衡时,布洛芬拆分反应的底物转化率接近50%,底物的对映体过量值达到94.3%,产物的对映体过量值则稳定在98%以上.而且固定化PEL催化拆分反应的周期缩短至12 h.同时,基于乒乓反应机制的动力学方程,建立了相应的拆分反应动力学模型,并得到了该模型的有关参数.In order to resolute racemic ibuprofen more effectively in the enzymatic reactor, uniform design was used for optimizing the reaction conditions of enzymatic resolution in BSTR. The result showed that the suitable concentration of ibuprofen, PEL and 1-propanol were 200 mg, 6 g and 0. 383 mL, respectively. Under this condition, the conversion of the reaction nearly reached 50% , the Veep reached more than 98% and the Vees reached 94.3%. It was also found that in BSTR, the reaction time was reduced to 12 h after optimization. Also, according to the mechanism of enzymatic esterification during the resolution of （R, S）- ibuprofen in nonaqueous media, the kinetic model of this enzymatic resolution was established.

关 键 词：均匀设计 脂肪酶 布洛芬 反应器 反应动力学 

分 类 号：Q93[生物学—微生物学]