瑞拉菌素产生菌GAO-1-GR-2发酵工艺条件优化研究  

Optimization of Fermentation Technology Conditions for Zuelamycin-Producing Strain GAO-1-GR-2

作　　者：程建坤[1] 安德荣[1] 高峰[1] 苟丽霞[1] 

机构地区：[1]西北农林科技大学植物保护学院陕西省农业分子生物学重点实验室,陕西杨凌712100

出　　处：《微生物学杂志》2009年第5期78-81,共4页Journal of Microbiology

基　　金：国家高技术研究发展计划(863计划;2007AA021503)

摘　　要：对瑞拉菌GAO-1-GR-2的发酵条件进行了单因素研究,通过正交试验设计对该菌株的培养基进行了筛选。并对其发酵条件进行了研究,结果表明:有机氮源以酵母粉和黄豆饼粉为宜;碳源以葡萄糖和饴糖最好,但考虑到发酵成本,应选用葡萄糖2%。发酵培养基应呈中性,以采用自然pH为宜,pH超过8,或小于7,则会抑制发酵产物活性;通气量为1:0.5最好,能降低20%耗氧量;摇床转速140 r/min,培养温度30℃,发酵周期72 h。抗生素发酵原粉效价能达到4 000μg/mL。Abstract The fermentation conditions of strain Ruila GAO-1-GR-2 were studied with single factor, and then its medium was screened through orthogonal experiment design. The results showed that yeast powder and soybean meal were suitable for organic nitrogen sources; glucose and maltose were the best carbon sources; however, taking the cost of fermentation into consideration, 2% glucose was the best alternative. And the medium should be neutral, and adopt it at natural pH ; pH over 8 or below 7 will inhibit the activity of fermentation product. The optimal ventilation was at 1 ： 0.5 which could reduce 20% of oxygen consumption; rpm of shaker was at 140, culture temperature at 30℃ ,with fermentation period for 72 hours. With these conditions the valence of the original powder of fermentation could reach up to 4 000 μg,/mL.

关 键 词：链霉菌 发酵 农用抗生素 瑞拉菌素 效价 

分 类 号：R978.12[医药卫生—药品]