一种短指软珊瑚Sinularia sp.克生活性筛选及其低极性成分GC/MS分析  

The Allelopathic Activity Evaluation and the GC/MS Analysis of the Low Polar Components from a Soft Coral Sinularia sp.

作　　者：王长云[1] 刘海燕[1] 孙雪萍[1] 李秀保[2] 郑彩娟[1] 韩磊[1] 邵长伦[1] 管华诗[1] 

机构地区：[1]中国海洋大学医药学院海洋药物教育部重点实验室,山东青岛266003 [2]中国科学院南海海洋研究所,广东广州510000

出　　处：《中国海洋大学学报（自然科学版）》2009年第5期930-932,共3页Periodical of Ocean University of China

基　　金：国家自然科学基金项目(40776073);国家高技术研究发展计划专项(908-01-ST12908-02-05-04);国家科技基础性工作专项(2007FY210500);国家教育部科技创新工程重大项目培育资金(706038);国家教育部新世纪优秀人才支持计划(NCET-05-0600)资助

摘　　要：采用卤虫致死和细胞毒活性筛选模型,对1种中国南海短指软珊瑚Sinularia sp.的乙醇提取物及各有机相进行了克生活性测定。结果表明,克生活性物质主要集中在石油醚相中。采用GC/MS技术,对石油醚相进行了分析,鉴定了15个化合物,主要为脂类和甾体类化合物;从石油醚相的甾醇部分鉴定了6个化合物,主要为麦角甾醇衍生物,占总甾醇含量的75.7%。推测甾醇类化合物是短指软珊瑚Sinularia sp.的主要克生活性物质。The allelopathic effects of the ethanol extract and its organic fractions of a soft coral Sinularia sp. from the South China Sea were evaluated by the model of lethality to brine shrimp Arternia salina and cytotoxicity. It indicated that the allelopathic substances were mainly in the petroleum ether fraction. The components in the petroleum ether fraction were analyzed by GC/MS, and 15 compounds were identified, most of which were lipids and steroids. From the sterol component（SC-P-1） isolated from petroleum ether fraction, six sterols were also identified by GC/MS to be ergosterol derivatives and as main constituents accounting for 75.7 % of SC-P-1. It was deduced that the sterols should be the main allelopathic substances of the soft coral Sinularia sp.

关 键 词：软珊瑚 短指软珊瑚 克生活性 甾醇类 GC/MS 

分 类 号：R914[医药卫生—药物化学]