联用莪术油和丝裂霉素对肝肿瘤细胞株SMMC-7721的作用研究  

Role of CCAO-MS in inhibiting the growth of human carcinoma cells SMMC-7721

作　　者：杨娟[1] 周玲玲[1] 袁冬平[1] 许立[1] 陈长华[1] 

机构地区：[1]南京中医药大学药理教研室江苏省中药药效与安全性评价重点实验室,江苏南京210029

出　　处：《安徽医药》2009年第11期1327-1329,共3页Anhui Medical and Pharmaceutical Journal

基　　金：江苏省中医药局指导性课题(NoHL07078)

摘　　要：目的探讨复方莪术油微球对肝癌细胞株的抑制作用及其作用机制。方法采用莪术油和丝裂霉素微球制备方法联用莪术油和丝裂霉素;采用MTT法检测莪术油和丝裂霉素单用和联用对肝癌细胞株SMMC-7721生长的抑制作用,并用流式细胞仪检测两药单用和联用对肝癌细胞增殖周期和凋亡的影响。结果MTT结果表明,单用和联用莪术油和丝裂霉素均可抑制肝癌细胞株SMMC-7721的生长,但联用莪术油和丝裂霉素采用减半剂量抑瘤率仍明显高于单用莪术油和丝裂霉素;流式细胞仪检测结果表明,莪术油和丝裂霉素合用可增强对肿瘤细胞增殖的抑制和促进凋亡的发生,较单用莪术油和丝裂霉素有增效作用。结论联用莪术油和丝裂霉素对肝癌细胞株SMMC-7721的生长抑制有增效作用,而诱导凋亡和阻滞细胞周期可能是其增效的重要机制。Aim To investigate the inhibition mechanism of CCAO-MS on inhibiting the growth of human carcinoma cells SMMC-7721.Methods The inhibiting effect was detected by MTT（monotetrazoluim） and the cells cyclic retardation and apoptosis rate were examined by using flowcytometry.Results MTT test showed that the inhibition mechanism of CCAO-MS on inhibiting the growth of human carcinoma cells SMMC-7721 is stronger than that of CAO or MMC only.The result of fluorescence microscope showed that CCAO-MS could induce hepatocell apoptosis and block the cell cycle. Conclusion CCAO-MS can inhibit the growth of hepatocell SMMC-7721. Inducing apoptosis and blocking the cell cycle are the important mechanism of inhibiting effect .

关 键 词：复方莪术油微球 肝癌 细胞凋亡 细胞周期 

分 类 号：R735.7[医药卫生—肿瘤] R737.14[医药卫生—临床医学]