成人正常肝和肝细胞癌DNA干系倍体的组织原位分析  

Analysis of DNA stem line ploidy heterogeneity of the normal hepatocyte and the hepatocellular carcinoma in the tissue section

作　　者：李朋军[1] 夏潮涌[1] 覃莉[1] 潘运龙[2] 

机构地区：[1]暨南大学医学院 [2]暨南大学附属第一医院,广东广州510632

出　　处：《中国体视学与图像分析》2009年第3期279-282,共4页Chinese Journal of Stereology and Image Analysis

基　　金：广东省自然科学基金项目(No.010367)

摘　　要：目的分析成人正常肝和肝细胞癌DNA干系倍体及细胞核形态学参数的变化。方法：选取15例成人正常肝和45例肝细胞癌患者的归档蜡块,分别制备4μm、10μm的连续组织学切片,利用TIGER 920G1细胞图像分析仪测量DNA干系倍体值及细胞核形态学参数。结果（1）成人正常肝DNA干系倍体主要以二倍体（73%）为主。（2）肝细胞癌的DNA指数在0.86~9.32之间。45例肝细胞癌病例中DNA干系倍体异质性率为77.8%,其中,34例（75.6%）为DNA干系非整倍体肿瘤,11例（24.4%）为近四/八/十六倍体肿瘤,无1例为DNA干系二倍体肿瘤。结论细胞核DNA干系倍体和细胞核形态学参数分析可作为鉴别人正常肝和肝细胞癌的参考指标。Objective To compare and analyze DNA stem line ploidy and morphological parameters of the normal Liver; specimen and the hepatoeellular carcinoma in adult. Methods 15 cases normal Liver; specimen and 45 heptoeellular carcinoma ones were selected, the serial slices of 4 μm and,10 μm of two tissues were prepared respectively. DNA stem line ploidy and its morphologieal parameters were analyzed with TIGER 920G image eytometry. Results （ 1 ） The major DNA stem line ploidy in normal human hepatocyte was diploid （73%）. The values of DNA stem line ploidy, mean volume of the karyon and dikaryon rate in the hepatocellular carcinoma patients were different statistically from those of the normal. （2） The DI of hepatocellular carcinoma was different from 0.86 to 9.32. The heterogeneity rate of DNA stem line ploidy of the 45 hepatoeellular carcinoma was 77.8%. The rate of DNA stem line aneuploidy was 75.6% , and none of them was DNA stem line diploidy tumor. Conclusion Analysis on DNA stem line ploidy and the morphological parameters of kaiyon are important indexes to identify the hepatocellular carcinoma from the normal.

关 键 词：肝细胞癌 DNA干系倍体 细胞图像光度术 形态学 

分 类 号：R735.7[医药卫生—肿瘤] R361.3[医药卫生—临床医学]