β分泌酶抑制剂对岗田酸(OA)诱发PC12细胞损伤的保护作用  

THE PROTECTIVE EFFECTS OF β-SECRETASE INHIBITOR ON INJURY OF PC12 CELLS INDUCED OF OA

作　　者：史晓东[1] 俞春江[1] 梁庆成[1] 

机构地区：[1]哈尔滨医科大学附属第二医院神经内科,黑龙江哈尔滨150001

出　　处：《牡丹江医学院学报》2009年第5期35-38,共4页Journal of Mudanjiang Medical University

摘　　要：目的:研究β分泌酶抑制剂对岗田酸(OA)诱发PC12细胞损伤的保护作用。方法:将培养的PC12细胞分为3组:正常对照组、OA损伤组和药物保护组。OA损伤组用OA处理细胞,药物保护组在用OA处理前30min加入β分泌酶抑制剂。采用MTT法、免疫组化法观察β分泌酶抑制剂的保护作用,Western blot检测全长APP和β-C末端片段(β-C-terminalfragment,βCTF)。结果:药物保护组与OA损伤组相比细胞数显著增多,受损变圆的细胞数较少;OD490值显著升高(P<0.01)。OA诱导PC12细胞内全长APP和βCTF含量增加,β分泌酶抑制剂进一步增加细胞内全长APP含量,并减少βCTF含量。结论:β分泌酶抑制剂对岗田酸(OA)诱发PC12细胞损伤有保护作用,其机制可能和β分泌酶抑制剂减少βCTF水平有关。Objective：To study the protective effects of β - secretase inhibitor on injury of PC12 ceils induced by okadaic acid （OA）. Methods：Culturing cells in medium with OA. β -secretase inhibitor as added in the medium. Morphological observation, MTT assays were used to study the protective effects of β -secretase inhibitor. APP, βCTF（ β -C -terminal fragment）were determined by Western blot method. Results： β - secretase inhibitor attenuated round shaped cells induced by OA, increased OD490, Owing to OA treatment, APP and βCTF level increased in PC12 cells. β - secretase inhibitor increased APP level furthermore but decreased βCTF. Conclusion：β - secretase inhibitor prevents PC12 cells from injury by OA and the mechanism may be related to the decrease of the level of βCTF.

关 键 词：PC12细胞 β分泌酶抑制剂 β-C末端片段 

分 类 号：R74[医药卫生—神经病学与精神病学]