黄芪苷Ⅳ对血管紧张素Ⅱ诱导新生大鼠心肌细胞肥大的保护作用  

Protective effects of astragaloside Ⅳ on hypertrophy induced by angiotensinⅡ in primary cultured cardiomyocytes of neonatal rats

作　　者：周红敏[1] 晋玉章[2] 谢文利[3] 刘艳霞[1] 

机构地区：[1]天津医科大学药理学教研室,天津300070 [2]武警医学院机能实验中心 [3]武警医学院药理学教研室,天津300162

出　　处：《中国药理学通报》2009年第10期1330-1334,共5页Chinese Pharmacological Bulletin

基　　金：教育部高校骨干教师资助计划基金项目(No2000GYD-6)

摘　　要：目的研究黄芪苷Ⅳ(astragalosideⅣ,AST)对血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)诱导新生大鼠心肌细胞肥大的保护作用并探讨其作用机制。方法AngⅡ刺激新生大鼠心肌细胞,制备心肌细胞肥大模型,分别用AST10mg·mL-1和20mg·mL-1进行预防性治疗。检测心肌细胞直径及细胞总蛋白含量,测定心肌细胞[Ca2+]i、肌浆网钙泵(SERCA)活力和钙调神经磷酸酶(CaN)活性。结果AngⅡ组细胞直径增大,总蛋白含量增加,与对照组相比差异有显著性(P<0.01);AST10mg·mL-1和20mg·mL-1能够降低心肌细胞肥大程度(P<0.05或P<0.01)、[Ca2+]i(P<0.01)及CaN活性(P<0.05或P<0.01)、提高SERCA活力(P<0.01)。结论AST对AngⅡ诱导心肌细胞肥大有明显的保护作用,可能与其降低[Ca2+]i、提高心肌SERCA活力及降低CaN活性有关。Aim To investigate the protective effects and mechanism of astragalosideⅣ on hypertrophy in-duced by angiotensin Ⅱ （ Ang Ⅱ ） in primary cultured cardiomyocytes of neonatal rats. Methods Cardiomyo-cytes were incubated with Ang Ⅱ to establish the hy- pertrophy model of primary cultured cardiomyocytes in neonatal rats. AST 10 mg · L^-1 or 20 mg · L^-1 were added with Ang Ⅱsimultaneously to observe its protective effects on cardiomyocytes. The diameter and the total protein content of cardiomyocyte were measured. The intracellular free calcium concentration（ [Ca2＋ ]i）, activity of sarcoplasmic reticulum Ca2＋ -ATPases（ SERCA） and activity of calcineurin （CaN） were determined. Results Compared with control group, diameter and total protein content of cardiomyocyte induced by Ang Ⅱ were increased byAST10 mg· L^-1 significantly, which were inhibited i and 20 mg · L ^- 1, respectively （ P〈 0. 05 or P 〈0. 01 ）. [ Ca2＋ ]i was decreased （ P 〈 0.01, respectively）, the activity of SERCA was increased （P 〈 0.01, respectively）and the activity of CaN was decreased（P 〈0. 05 or P 〈0. 01 ） by AST 10 mg · L^-1 and 20 mg· L ^1, respectively. Conclusion Primary cultured cardiomyocytes of neonatal rats are protected by AST from hypertrophy induced by Ang Ⅱ by decreasing [ Ca2 ＋ ]i, increasing the activity of SERCA and inhibiting the activity of CaN.

关 键 词：黄芪苷Ⅳ 血管紧张素Ⅱ 心肌肥大 游离钙离子浓度 心肌肌浆网钙泵 钙调神经磷酸酶 原代培养 

分 类 号：R-332[医药卫生] R284.1