蛋白激酶C-βⅡ对SMMC-7721人肝癌细胞增殖的影响  

Effect of Protein Kinase Cβ Ⅱ on the Proliferation of Human Hepatoma Cells

作　　者：刘锋[1] 王琳[1] 胡明道[1] 周玉凯[1] 

机构地区：[1]昆明医学院第二附属医院肝胆外科,云南昆明650101

出　　处：《昆明医学院学报》2009年第10期1-4,9,共5页Journal of Kunming Medical College

基　　金：国家自然科学基金资助项目(30660184);云南省自然科学基金资助项目(2006C0057Q);云南省中青年学术技术带头人后备人才基金资助项目(2008PYD18)

摘　　要：目的研究蛋白激酶C-βⅡ对SMMC-7721人原发性肝癌细胞增殖的影响.方法体外基因扩增获得pcDNA3/PKCβⅡ真核表达质粒.脂质体介导pcDNA3/PKCβⅡ基因体外转导人原发性肝癌SMMC-7721细胞,分为实验组(pcDNA3/PKCβⅡ)、对照组(pcDNA3空载体)及空白对照组.细胞计数、MTT法、细胞形态学观察、流式细胞仪等方法检测PKCβⅡ对SMMC-7721人原发性肝癌细胞增殖的影响.结果细胞生长曲线检测结果显示转导入PKCβⅡ的SMMC-7721细胞数目明显增加;MTT法显示转染PKCβⅡ的人原发性肝癌细胞SMMC-7721的OD值增加(P<0.01);细胞形态学观察实验组SMMC-7721细胞间连接紧密,巨核、双核、多核、奇异型的核也明显增加,核分裂像增多,特别是出现不对称、多极性及顿挫性等病理性核分裂像尤为明显;流式细胞仪检测显示转导PKCβⅡ的SMMC-7721细胞细胞周期中S期细胞明显增多.结论PKCβⅡ可促进SMMC-7721细胞增殖,其机制可能与促进细胞DNA合成相关.Objective To study the effect of PKC β Ⅱ on the proliferation of human hepatoma ceils. Methods SMMC-7721 hepatoma cells were cultured in vitro, pcDNA3/PKC β Ⅱ was transducted into the SMMC-7721 by lipofection. We studied the effect of PKC β Ⅱ on increasing cell growth through cell counting, cell morphology observing, MTT detection and flow cytometry detection. Results The cell growth curve showed that the number of SMMC-7721 cells transdueted with pcDNA3/PKC β Ⅱ gradully increased, the MTF detection displayed that the OD value of SMMC-7721 cells with pcDNA3/PKC β Ⅱ transduction significantly increased （P 〈 0.01 ）,cell morphology observation showed that mitotic figure, such as asymmetry, multi-polarity and abortive pathology is particularly evident in cells with pcDNA3/PKC β Ⅱ transduction, the results of flow cytometry showed that the PKC β Ⅱ transfeetion increased the cell number in S phase. Conclusions The pcDNA3/PKC β Ⅱ transduetion may promote the growth of SMMC-7721 hepatoma cells, and the mechanism may he promoting DNA synthesis.

关 键 词：蛋白激酶CβⅡ 人原发性肝癌 细胞增殖 

分 类 号：R735.7[医药卫生—肿瘤]