Orphan nuclear receptor Nur77 is required for the differentiation of C6 glioma cells induced by cholera toxin  

作　　者：Dong XU Yi-jun HUANG Yan LI Wei YIN Guang-mei YAN 

机构地区：[1]Departments of Pharmacology and Guangzhou 510089, China [2]Biochemistry, Zhong-shan Medical College, Sun Yat-Sen University, Guangzhou 510089, China

出　　处：《Acta Pharmacologica Sinica》2009年第11期1543-1549,共7页中国药理学报（英文版）

基　　金：Acknowledgements Project was supported by National Natural Science Foundation of China, Key program （No 30830111）.

摘　　要：Aim： To investigate a possible regulator gene involved in the cholera toxin-induced differentiation of rat C6 glioma cells. Methods： The global changes in the mRNA expression pattern induced by cholera toxin were analyzed using gene chip microarray. The selected gene was then silenced by RNA interference or overexpressed with an ORF plasmid to determine its necessity in this process Results： Nur77, a member of the orphan nuclear receptor family （NR4A）, was markedly up-regulated during the process of differentiation. Furthermore, RNAi of nur77 attenuated the induction effect of cholera toxin on C6 cells, whereas overexpression of nur77 led to similarly differentiated behavior, including morphologic and biomarker changes, as well as cell cycle arrest. Conclusion： Nur77 participated actively and essentially as an important regulator in the cholera toxin-induced differentiation of C6 cells.Aim： To investigate a possible regulator gene involved in the cholera toxin-induced differentiation of rat C6 glioma cells. Methods： The global changes in the mRNA expression pattern induced by cholera toxin were analyzed using gene chip microarray. The selected gene was then silenced by RNA interference or overexpressed with an ORF plasmid to determine its necessity in this process Results： Nur77, a member of the orphan nuclear receptor family （NR4A）, was markedly up-regulated during the process of differentiation. Furthermore, RNAi of nur77 attenuated the induction effect of cholera toxin on C6 cells, whereas overexpression of nur77 led to similarly differentiated behavior, including morphologic and biomarker changes, as well as cell cycle arrest. Conclusion： Nur77 participated actively and essentially as an important regulator in the cholera toxin-induced differentiation of C6 cells.

关 键 词：cholera toxin malignant glioma DIFFERENTIATION orphan nuclear receptor nur77 

分 类 号：Q813[生物学—生物工程] S852.4[农业科学—基础兽医学]