AB-8大孔树脂对槐花总黄酮吸附性能研究  

Macroporous Resin AB-8 Adsorption for Purifying Total Flavonoids from Pagoda Flowers

作　　者：王慧彦[1] 方芸[1] 王妍[1] 杨树平[1] 张珍明[1] 张所信[1] 

机构地区：[1]淮海工学院,江苏连云港222005

出　　处：《食品科学》2009年第20期146-150,共5页Food Science

基　　金：淮海工学院校内立项课题(KX07041)

摘　　要：目的:探讨AB-8大孔树脂对槐花中总黄酮静态和动态的吸附性能。方法:用微波辅助法得到粗提液,利用分光光度法测定样品中黄酮含量,考察吸附剂用量、粗提液浓度、pH值、吸附时间等条件对吸附结果的影响。结果:AB-8树脂对槐花中总黄酮具有较好的吸附性能。静态吸附最佳条件:树脂与提取液比为1:20(g/ml)、25℃、pH5～6、粗提液中的黄酮含量0.10～0.17mg/ml、恒温振荡70min、70%乙醇洗脱,在此条件下,黄酮总回收率为71.94%;动态吸附的最佳条件为:提取液上样质量浓度0.045～0.070mg/ml、pH5、以0.2ml/min的流速通过径高比为1:8的层析柱,70%的乙醇4BV洗脱,在此条件下,黄酮总回收率为39.32%。结论:AB-8树脂较适于分离纯化槐花总黄酮。This work studied static and dynamic adsorption and desorption of total flavonoids prepared from pagoda flowers by microwave-assisted extraction on macropurous resin AB-8. Results showed that AB-8 resin had good adsorption capacity to pagoda flower flavonoids; the optimum static adsorption and desoprtion conditions were as follows： adsorption on a shaking table for 80 min at 25 ℃ with a ratio of resin to sample 1：20（g/ml）, at pH 5 --6, sample concentration 0.10--0.17 mg/ml and then desorption with 70% aqueous ethanol as eluent, and under such conditions, the recovery of flavonoids was 71.94%; a recovery of total flavonoids of 39.32% was obtained by the following optimum dynamic adsorption and desoption conditions of sample concentration 0.045--0.070 mg/ml, pH 5, ratio of column diameter to length 1：8, flow rate 0.2 ml/min and 70% aqueous ethanol as eluent.

关 键 词：大孔树脂 槐花 总黄酮 吸附 

分 类 号：Q946.86[生物学—植物学]