拟南芥中1个新snoRNA基因的鉴定  

Identification of a Novel snoRNA Gene in Arabidopsis thalianan

作　　者：徐玲[1] 胡娜[1] 刘仁荣[1] 裘雪梅[1] 

机构地区：[1]江西科技师范学院生命科学学院,江西南昌330013

出　　处：《安徽农业科学》2009年第33期16255-16256,16281,共3页Journal of Anhui Agricultural Sciences

基　　金：江西科技师范学院自然科学立项课题(KY2008ZY03);江西省高等学校教学研究省级立项课题(JXJG-13-29)

摘　　要：[目的]鉴定拟南芥中1个新snoRNA基因。[方法]运用生物信息学方法筛选拟南芥基因组序列,并对候选的基因序列结构、基因组织形式和功能进行分析。[结果]在拟南芥基因组中发现snR95box H/ACAsno RNA上游有一段序列具有典型boxC/DsnoRNA的保守组件和结构特征,并具有2段与rRNA互补、长度超过数10个核苷酸的反义序列,该基因下游的反义序列与snoRNA数据库中水稻Z270的下游反义序列一致,命名为boxC/Dsno RNA-AthZ270。[结论]拟南芥Z270 snoRNA具有不同与一般snoRNA的功能。[ Objective ] To identify a novel snoRNA gene in Arabidopsis thalianan. [ Method] Genome sequence of Arabidopsis thalianan was screened by using bioinformatics methods, and the sequence structure, organization form and function of typical candidate gene were analyzed. [ Result] The identified snR95 box H/ACA snoRNA had conservative component and structural features of box C/D snoRNA family, possessed two more than 10 nt long rRNA antisense elements. The result revealed that the novel snoRNA is a partial counterpart of the rice Z270, named box C/D snoRNA-AthZ270. [ Conclusion] Z270 snoRNA in Arabidopsis thalianan has different function with common snoRNA.

关 键 词：拟南芥 SNORNA AthZ270 

分 类 号：Q522[生物学—生物化学]