三格厌氧氧化槽处理农村生活污水的菌种筛选及条件选择  

Study on Bacteria-screening for the Treatment of Rural Wastewater with Triple-vessel Anaerobic Oxidation Tank and Its Best Condition

作　　者：江晨舟[1] 徐功娣[1,2] 赵晓祥[1] 

机构地区：[1]东华大学环境科学与工程学院,上海201620 [2]齐齐哈尔大学化工学院,黑龙江齐齐哈尔161006

出　　处：《安徽农业科学》2009年第33期16507-16509,共3页Journal of Anhui Agricultural Sciences

基　　金：上海市科委"科技创新行动计划"项目(07dz12408);上海市重点学科建设项目(B604);上海市博士后科学基金资助项目(08R214102);齐齐哈尔市科委资助项目(SFG06-30)

摘　　要：[目的]为农村生活污水的净化处理提供菌源和技术支持。[方法]从生物净化器/强化浮床组合工艺处理农村生活污水工程的三格厌氧氧化槽中提取菌种,并在筛选培养基中复筛出3株高效活性菌株。经过生理生化试验和优化条件选择试验筛选出优化菌株。[结果]将3槽内菌株富集后各自初步筛选出3株菌株A-1、A-2、A-3,B-4、B-5、B-6,C-7、C-8、C-9。在一定培养条件下根据总氮、总磷和COD的变化筛选出高效菌株A-1、B-5、C-7作为优化试验的菌种。A-1、B-5、C-7的1∶1∶1混合培养菌株D-X因单株之间的协同作用使其脱氮除磷速率比单株都高,故选用D-X作为生物脱氮除磷的接种剂。经优化,D-X的最佳培养条件:在300℃和厌氧条件下,pH值为7.0左右,N/P为6,接种量为20%。[结论]优选出了混合培养菌株D-X,该菌株脱氮除磷效果明显,可广泛用于农村生活污水的处理。[ Objective] The technical support and the bacteria sources for the purification of life sewage in rural were provided. [ Method ] The bacteria source extracted from triple-vessel anaerobic oxidation tank for the treatment of the domestic sewage in rural area was purified with the technical combination of biological purification instillation and floating-bed. 3 strains with high activity were isolated from the media. The optimal strain was selected through the physiological and biochemical test and optimal test condition. [ Results] 3 strains of A-1, A-2, A-3, B-4, B-5, B-6, C-7, C-8 and C-9 were preliminarily screened out from 3 tanks where the strains were accumulated, respectively. The highperformance strains： A-1, B-5 and C-7 were selected according to total nitrogen, total phosphorus and COD under certain culture condition. The D-X of the mixed culturing strain of A-1, B-5, C-7 （ 1 ： 1 ： 1 ） had the higher rate of nitrogen and phosphorus removal than these of single strain because of their synergy. Thus, the D-X of the mixed strain could be selected as the inoculants of biological nitrogen/phosphorus removal. After optimized, the best culturing condition of D-X was ： the temperature, 300 ℃ ; the environment, anaerobic culture ; the pH value, about 7.0; the rate of N/P, 6 and the inoculation amount, 20%. [ Conclusion] The D-X of mixed strain was best with obvious effect on the nitrogen and phosphorus removal, which could be widely used in the treatment of domestic sewage in rural area.

关 键 词：菌种筛选 优化选择 生物脱氮除磷 

分 类 号：X703[环境科学与工程—环境工程]