牙周致病菌和致龋菌间生长关系的体外动态观察  

The ecological relationship of periodontal pathogens and cariogenic bacteria in plaque biofilm and suspension

作　　者：王岷峰[1,2,3] 李德懿[1,2] 李宗林[4] 

机构地区：[1]上海交通大学医学院附属第九人民医院.口腔医学院 [2]上海市口腔医学研究所,200011 [3]首都医科大学口腔医学院牙周粘膜科 [4]复旦大学生命科学院遗传工程实验室

出　　处：《北京口腔医学》2009年第5期244-248,共5页Beijing Journal of Stomatology

基　　金：国家自然基金资助项目(30070813)

摘　　要：目的研究6种代表性牙周致病菌和致龋菌在菌斑生物膜和悬浮液中的消长关系。方法将双菌组(致龋菌+牙周致病菌)接种于改良恒化器中,连续培养1h、24h、48h和96h,然后取在羟基磷灰石表面形成的生物膜和悬浮液再进行细菌培养。结果与血链球菌混合培养时,菌斑生物膜和悬浮液中牙周致病菌均增多,而血链球菌明显减少(P<0.05);具核梭杆菌与变形链球菌培养时,菌斑生物膜和悬浮液中具核梭杆菌明显增多,而变形链球菌减少(P<0.05)。菌斑生物膜中致龋菌占优势,相应的悬浮液中牙周致病菌96h开始占优势。悬浮液中细菌量波动更明显。结论2类致病菌在菌斑生物膜与相应的悬浮液中的生长是不一致的。2类致病菌之间相互作用也许是决定菌斑生物膜内部生态环境发展方向的主要因素。Objective To investigate the relationship of six representative periodontopathic and cariogenic bacteria in plaque biofilm and suspension. Methods Six periodontopathic and cariogenic bacteria of P. gingivalis, A. actinomycetemcomitans, F. nucleatum, S. sanguis, A. viscosus and S. mutans were in pair cultured in modified MD-300 ehemostat for 1 h,24 h ,48 h and 96 h respectively, and then the plaque biofilm removed from the hydroxyapatite disks and the suspension at each time point were cultured again. Results When cultured with S. sanguis, respectively, the bacterial number of periodontal pathogens in biofilm and suspension increased and S. sanguis reduced （P 〈 0. 05 ） . When F. nucleatum was cultured with S. mutans, the F. nucleatum in biofilm and suspension increased and S. mutans decreased （P 〈 0. 05）. Cariogenic bacteria predominated in biofilms and periodontal pathogens started to dominate in suspension at 96 h. The bacterial number in suspension fluctuated more obviously than in biofilm. Conclusion The growth of periodontopathic and eariogenic bacteria in biofilms was not identical to that in corresponding suspension. The interaction between two kinds of oral pathogenic bacteria was a major contributing factor in determining the shift of the ecological environment in plaque biofilm.

关 键 词：牙周致病菌 致龋菌 菌斑生物膜 悬浮液 

分 类 号：R780.2[医药卫生—口腔医学]