食管鳞癌细胞HIF—1α和COX-2表达及肿瘤组织微血管密度的临床意义研究  

Expressions of HIF-1α and COX-2 in esophageal squamous cell carcinoma and their correlation with intratumoral MVD

作　　者：郭隆佳[1] 陈志坚[1] 陈创珍[1] 洪超群[2] 吴晓[2] 

机构地区：[1]广东省汕头大学医学院附属肿瘤医院放疗科,汕头515031 [2]广东省汕头大学医学院附属肿瘤医院病理科,汕头515031

出　　处：《中华放射医学与防护杂志》2009年第5期502-505,共4页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的研究食管鳞癌细胞HIF-1α和COX-2的表达和肿瘤微血管密度（MVD）与食管癌放疗疗效的关系。方法应用免疫组织化学二步法对55例食管鳞癌食管镜活检组织中HIF-1α、COX-2的表达进行检测，并用同样方法检测癌组织中CD34的表达评估MVD。分析其与临床病理学参数及放疗疗效的关系。结果HIF-1α和COX-2的阳性表达率分别为92．7％和69．1％，MVD为2～29条／高倍视野，平均为（11．2±4．1）条。HIF-1α和COX-2的表达水平与MVD数量无关。HIF-α和COX-2的表达水平与肿瘤的细胞分化程度、肿瘤部位均无关。单因素分析示食管癌放疗后近期疗效中位生存期和2年生存率与HIF-1α、COX-2表达及MVD无关。结论食管鳞癌组织中HIF-1α和COX-2的表达情况和肿瘤组织中微血管密度无关。HIF-1α和COX-2的表达水平及MVD均不能作为食管鳞癌放射治疗的预后指标。Objective To investigate the expressions of HIF-1α, COX-2 and introtumoral mierovascalar density（MVD） in esophageal squamous cell carcinoma （ESCC）, and their impacts on the efficacy of radiotherapy. Methods Expressions of HIF-1α and COX-2 were assessed by immunohistoehemistry methods in fifty-five cases of ESCC. Microvessels were labelled in sections of same tissues by CD34 immunohistoehemistry. The correlations with clinicopathological features and postradiotherapy survival rates were evaluated and analyzed. Results The MVD ranged from 2 to 29 （ mean of 11.2 ± 4.1 ）. The positive rates of HIF-1α and COX-2 protein expression were 92.7 % and 69.1%, respectively, both of which were not correlated with intratumoral MVD. The expressions of HIF-1α and COX-2 were not correlated with histological grade or the location of the primary tumor. Univariate analysis revealed that 2-year survival rates and median survival time following radiotherapy were not assocaited with the expressions of HIF-1α, COX-2 or MVD. Conclusions MVD and the expressions of HIF-1α and COX- 2 in ESCC are not efficient to predict the outcome of ESCC following radiotherapy.

关 键 词：食管肿瘤 HIF-1Α COX-2 微血管密度 放射治疗 预后 

分 类 号：R735.1[医药卫生—肿瘤]