氯化钴化学模拟低氧对牙周膜成纤维细胞增殖及凋亡的影响  

Influence of cobalt chloride-induced hypoxia on the proliferation and apoptosis of periodontal ligament cells

作　　者：宋忠臣[1] 束蓉[1] 李希庭[1] 胡景超[1] 张秀丽[2] 

机构地区：[1]上海交通大学医学院附属第九人民医院·口腔医学院牙周科上海市口腔医学重点实验室,上海200011 [2]上海市口腔医学研究所,上海市口腔医学重点实验室,上海200011

出　　处：《上海口腔医学》2009年第5期489-492,共4页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30801292);上海市重点学科建设项目(S30206);上海市高校优秀青年教师科研专项基金(JDY07059)~~

摘　　要：目的:研究低氧对人牙周膜成纤维细胞(periodontal ligament cells,PDLCs)的增殖及低氧诱导因子1α(hypoxia-inducible factor-1α,HIF-1α)和Caspase-3表达的影响。方法:采用二氯化钴(cobalt chloride,CoCl2)模拟低氧作用于人PDLCs,用MTT法检测人PDLCs的活力,并通过荧光定量PCR和Western印迹观察低氧处理后人PDLCs中HIF-1α和Caspase-3的表达变化。采用SAS6.12软件包对所得数据进行统计学分析。结果:低氧使人PDLCs存活率降低,并呈浓度和时间依赖;低氧促进HIF-1α蛋白的表达,而HIF-1α mRNA的水平未见影响(P>0.05);低氧使Caspase-3 mRNA及Caspase-3蛋白表达均上调。结论:低氧抑制人PDLCs增殖,这与低氧诱导HIF-1α表达,促进细胞凋亡有关。提示低氧在牙周炎发生和发展中起一定调控作用。PURPOSE： To investigate the effect of hypoxia on proliferation and expression of HIF-1α and Caspase-3 in human periodontal ligament cells （PDLCs）. METHODS： Human PDLCs were exposed to cobalt chloride in order to mimic hypoxia. Cell viability of PDLCs was determined by MTT methods. Expression of HIF-1α and Caspase-3 was measured by real time PCR and Western blot. The data was statistically analyzed with SAS6.12 software package for one-way ANOVA. RESULTS： Cell viability of PDLCs significantly decreased when exposed to hypoxia in a time- and dose-dependent manner. Hypoxia induced the expression of HIF-1α,up-regulated the expression of Caspase-3. CONCLUSIONS： Hypoxia inhibits cell proliferation, which involves the expression of HIF-1α and Caspase-3, resulting in the production of the apoptosis. The results suggest that hypoxia may play a role in the induction and progression of chronic periodontitis. Supported by National Natural Science Foundation of China（Grant No.30801292）, Shanghai Leading Academic Discipline （Grant No.S30206） and Research Fund for Excellent Young Teachers of Shanghai Municipal College and University （Grant No.JDY07059）.

关 键 词：氯化钴 低氧 牙周膜成纤维细胞 HIF-1Α 凋亡 

分 类 号：R78[医药卫生—口腔医学]