钛表面TiO_2纳米孔对骨髓基质细胞黏附和铺展的影响  

The effects of TiO_2 nano-pore on titanium surface on cell adhesion and spreading of bone marrow stromal cells

作　　者：张梅[1] 黄其煜[2] 周晓健[1] 孙强[1,3] 杨菲[1] 陈万涛[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院·口腔医学院口腔颌面外科,上海市口腔医学重点实验室,上海200011 [2]上海交通大学微电子学院,上海200030 [3]郑州大学第一附属医院口腔颌面外科,河南450000

出　　处：《上海口腔医学》2009年第5期493-498,共6页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30572053);上海市纳米科技专项(0452nm063);上海市科学技术委员会资助项目(09DZ2271100);上海市重点学科建设项目(S30206)~~

摘　　要：目的:体外研究钛表面TiO2纳米孔层结构对骨髓基质细胞(bone marrow stromal cells,BMSCs)的黏附、铺展形态等生物学特性的影响。方法:采用电化学阳极氧化法和化学腐蚀技术,在纯钛表面制备出特定尺度和形貌的TiO2纳米孔层结构,以猪BMSCs为研究对象,应用激光共聚焦显微镜、扫描电子显微镜等技术检测细胞黏附、铺展形态和骨架蛋白排列等生物学特性,采用SAS6.12软件包对数据进行t检验。结果:阳极氧化法和化学腐蚀技术制备的钛表面TiO2纳米孔层结构纳米尺度是孔径约为30nm,深度15nm。BMSCs接种于处理组材料与对照组材料4h和8h后,处理组钛片上细胞黏附数均显著大于对照组(P<0.05);在接种8h时,处理组材料表面细胞向多个方向伸出丰富细长伪足,细胞骨架蛋白粗大,铺展面积较大。对照组表面细胞伪足较少且粗短,细胞骨架蛋白细小,铺展面积较小;处理组细胞铺展面积是对照组的1.35倍(P=0.001)。结论:阳极氧化法和化学腐蚀技术制备的钛表面TiO2纳米孔层结构对BMSCs的黏附、铺展等生物学行为起到显著的促进作用。PURPOSE： To study the effect of TiO2 nano-pore on titanium surface on cell biological characteristics of bone marrow stromal cells （BMSCs）, such as cell adhesion and spreading. METHODS： TiO2 nano-pore structure on titanium surface with certain shape and size was prepared by electrochemical anodizing and chemical corrosion technologies. The effect of TiO2 nano-pore on titanium surface on cell adhesion, morphology, eytoskeleton protein of BMSCs was detected by laser scanning confoeal microscope, scanning electron microscope. The data was analyzed by Student t test using SAS6.12 software package. RESULTS： TiO2 nano-pores were produced on titanium surface, which was of about 30nm in width and 15nm in depth. More adhesive cells, larger cell spreading area, thinker cytoskeleton, richer fine pseudopodia were observed in the treatment group than in the control group after 4 hours and 8 hours of incubation of BMSCs （P〈0.05）. The area of BMSCs cell spreading in treatment group was 1.35 times than in the control group （P=0.001）. CONCLUSION： TiO2 nano-pores produced on titanium surface by electrochemical anodizing and chemical corrosion technologies may promote cell adhesion and spreading of BMSCs. Supported by National Natural Science Foundation of China （Grant No.30572053）,Nanometer Research Project of Shanghai Municipality （Grant No. 0452nm063）, Research Fund of Science and Technology Commission of Shanghai Municipality （Grant No. 09DZ2271100） and Shanghai Leading Academic Discipline Project （Grant No. S30206）.

关 键 词：骨髓基质细胞 生物学行为 二氧化钛 纳米结构 阳极氧化 

分 类 号：R783.1[医药卫生—口腔医学]