番茄提取物抑制乳腺癌生长的实验研究  

Experimental study of inhibitory effects of tomato extraction on breast cancer growth

作　　者：马文杰[1] 康欣梅[1] 张清媛[1] 

机构地区：[1]哈尔滨医科大学第三临床医学院内科,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2009年第5期441-442,447,共3页Journal of Harbin Medical University

基　　金：黑龙江省教育厅海外学人资助项目(1151hz032)

摘　　要：目的探讨番茄提取物在乳腺癌动物模型中对肿瘤增殖、凋亡及血管生成的影响。方法建立二甲基苯蒽诱导的大鼠乳腺癌模型,给予高中低剂量番茄提取物灌胃,应用5-溴-2-脱氧尿嘧啶核苷标记法检测细胞增殖;原位末端标记法检测细胞凋亡;免疫组化法检测肿瘤微血管密度。结果番茄组乳腺肿瘤的增殖指数和肿瘤微血管密度均低于对照组,且高剂量番茄组中乳腺肿瘤的凋亡指数较对照组升高,差异具有显著性(P<0.05)。结论番茄提取物在乳腺癌中具有抗增殖、促凋亡和抗血管生成活性,具有潜在的抗肿瘤应用价值。Objective To investigate the effeets of tomato extraction on tumor proliferation, apoptosis and angiogenesis in breast cancer animal model. Methods DMBA-induced breast caner models in SD rats were set up and received low-dose, middle-dose and high-dose tomato extraction by garage. Tumor cell proliferation was detected by bromouracil deoxyriboside labeling, cell apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling, and tumor microvascular density was detected by immunohistochemical method. Results The tumor proliferative index and microvaseular density were decreased in tomato group compared with control, and the apoptotic index was increased in high-dose tomato group. The difference was statistically significant（P 〈 0.05）. Conclusion The tomato extraction can inhibit tumor proliferation, promote apoptosis and decrease angiogenesis in breast cancer, and it has potential value for application in cancer treatment.

关 键 词：番茄 乳腺癌 凋亡 增殖 血管生成 

分 类 号：R737.9[医药卫生—肿瘤]