干细胞因子在胰腺星状细胞和肥大细胞黏附中的作用  

Effect of on adhesion between pancreatic stellate cell and mast cell

作　　者：吴恺[1] 徐刚[1] 徐铭益[1] 王兴鹏[2] 

机构地区：[1]上海交通大学附属第一人民医院消化科,上海200080 [2]同济大学附属第十人民医院

出　　处：《中华胰腺病杂志》2009年第5期318-320,共3页Chinese Journal of Pancreatology

基　　金：上海市卫生局课题资助（024016）

摘　　要：目的观察干细胞因子（SCF）在活化胰腺星状细胞（PSC）中的表达以及在PSC与肥大细胞黏附中的作用。方法采用SD大鼠胰腺组织块培养分离活化PSC，应用RT—PCR免疫细胞化学染色法检测PSC的SCFmRNA和蛋白的表达；经不同剂量的抗SCF抗体中和PSC表面SCF，采用β-氨基已糖苷酶测定观察肥大细胞与PSC之间的黏附；PSC经10ug/ml丝裂霉素C处理后为饲养层细胞，与肥大细胞共培养，硫堇兰染色观察培养后2、3、5d的肥大细胞生长情况。结果活化的PSC表达SCFmRNA和蛋白。对照组、1mg／ml和10mg／ml抗体处理组黏附的肥大细胞量分别为每视野（48±10）个、（28±8）个和（23±9）个，抗体组较对照组明显减少（P〈0．05）。1mg／ml和10mg／ml抗体组的肥大细胞黏附率分别为（53±3）％和（45±5）％。PSC和肥大细胞在无血清条件下共培养1d后，PSC有肥大细胞黏附；共培养3d后黏附的数量增加；5d后呈堆积状，有较多集落形成，PSC基本消失。结论活化的PSC可合成分泌SCF，引起肥大细胞的黏附和生长，Objective To investigate stem cell factor （SCF） expression in activated pancreatic stellate cell （PSC） and the effect of SCF on adhesion between PSC and mast cell （ MC ）. Methods PSC of SD rat was separated and activated by pancreatic tissue culture ; the expression of SCF mRNA and protein was detected by RT-PCR and immunochemistry. SCF was blocked with different dose of anti-SCF antibody, the rate of adhesion between PSC and MC was determined by thionin blue stain and β-hexasaminidase assay. PSC was stimulated by 10 ug/ml mitomycin-C and was co-cultured with MC, then the growth of MC was observed at 2, 3, 5 day, respectively. Results Activated PSC expressed SCF mRNA and protein, the number of adhered MC in the control, 1 mg/ml and 10 mg/ml antibody groups was 48 ± 10, 28 ± 8, and 23± 9, respectively; the difference between antibody and control group was statistically significant （ P 〈 0.05 ）. One day after PSC and MC was co-cultured without serum, there were MC adhesion; 3 days later the rate of adhesion increased; 5 days later the adhesion appeared bulk-like and lots of colonies were present, while PSC basically disappeared. Conclusions Activated PSC may synthesize, express SCF and induced adhesion and growth of MC.

关 键 词：干细胞因子 胰腺 星形细胞 肥大细胞 

分 类 号：R576[医药卫生—消化系统] R562.25[医药卫生—内科学]