放化疗抵抗的胰腺癌细胞中干细胞标志物的表达  

Expression of stem cell markers in pancreatic cancer cells resistant to chemoradiotherapy

作　　者：杜志勇[1,3] 魏翠凤[2] 田锐[1] 江建新[1] 王敏[1] 秦仁义[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院胆胰外科,武汉430030 [2]湖北省荆门市第一人民医院内分泌科 [3]上海交通大学医学院附属瑞金医院普外科

出　　处：《中华胰腺病杂志》2009年第5期321-323,共3页Chinese Journal of Pancreatology

基　　金：国家自然科学基金（30471693）

摘　　要：目的观察放化疗抵抗的胰腺癌细胞的Bcl-2、survivin及干细胞标志物Oct-4、ABCG2蛋白表达的变化，探讨这部分肿瘤细胞耐受放化疗的原因。方法采用同步放化疗干预人胰腺癌细胞系SW1990、BxPC3、pc3、jf305，获得放化疗抵抗的胰腺癌细胞，以不行放化疗处理的细胞作为对照。采用Western blotting检测它们的Bcl-2、survivin、Oct-4和ABCG2蛋白的表达变化。结果放化疗抵抗的SWl990、BxPC3、pc3、jt305胰腺癌细胞Bcl-2蛋白表达量分别为0．7955±0．0326、0．5718±0．0212、0．6137±0．0382和0．8733±0．0461；survivin蛋白表达量分别为0．8207±0．0490、0．6973±0．0211、0．7967±0．0346和0．8013±0．0398；Oct-4蛋白表达量分别为0．8728±0．0177、0．7861±0．0139、0．4794±0．0932和0．4216±0．1043；ABCG2蛋白表达量分别为0．7810±0．1370、0．4957±0．1126、0．6102±0．1358和0．4670±0．1274。对照组相应细胞系的4种蛋白表达量分别为0．4723±0．018、0．2954±0．0103、0．3587±0．0201和0．2718±0．0136；0．4717±0．0274、0．3587±0．0113、0．3891±0．0147和0．3326±0．0124；0．6053±0．0142、0．4236±0．0086、0．2385±0．0671和0．1985±0．0582；0．31564-0．0582、0．2360±0．0423、0．2813±0．0512和0．1808±0．0370。放化疗后胰腺癌细胞的4种蛋白表达量均显著增加（P〈0．05）。结论放化疗抵抗的胰腺癌细胞中可能富含肿瘤干细胞。Objective To investigate the expression of Bcl-2, survivin and pancreatic cancer stem cells markers Oct-4 and ABCG2 in pancreatic cancer cells resistance to chemoradiotherapy, and explore its mechanism. Methods Concurrent chemoradiotherapy was used to obtain pancreatic cancer cells resistant to chemoradiotherapy, the pancreatic cancer cells without chemoradiotherapy treatment were used as control. Western-blot was applied to detect the expression of Bcl-2, survivin, Oct-4, ABCG2. Results The expression of Bcl-2 was 0. 7955 ± 0. 0326,0 . 5718 ±0. 0212, 0. 6137 ±0. 0382 and 0. 8733 ±0. 0461, respectively; the expression of survivin protein was 0. 8207 ± 0. 0490, 0. 6973 ± 0. 0211, 0. 7967 ± 0. 0346 and 0. 8013 ± 0. 0398, respectively; the expression of Oct-4 protein was 0. 8728 ± 0. 0177, 0. 7861 ± 0. 0139, 0. 4794 ± 0. 0932 and 0. 4216 ± 0. 1043, respectively; the expression of ABCG2 protein was 0. 7810 ± 0. 1370, 0. 4957 ±0. 1126, 0. 6102 ± 0. 1358 and 0. 4670 ± 0. 1274, respectively, in resistant pancreatic cancer cells of SW1990, BxPC3, pc3, if305 cell line. The corresponding values in the control group were 0. 4723 ± 0. 018, 0.2954 s 0.0103, 0.3587 ± 0. 0201 and 0.2718 ± 0.0136 ; 0.4717 ± 0.0274, 0. 3587 ± 0.0113, 0. 3891 ± 0.0147 and 0. 3326 ± 0.0124 ; 0.6053 ± 0.0142, 0. 4236 ± 0. 0086, 0.2385 ± 0. 0671 and 0. 1985 ± 0. 0582 ; 0. 3156 ±0. 0582, 0.2360 ±0.0423, 0. 2813 ±0.0512 and 0. 1808 ±0. a0370. The expression of all the four proteins signifieantly increased after chemoradiotherapy （ P 〈 0. 05 ）. Conclusions Pancreatic cancer cells resistant to chemoradiotherapy may contain cancer stem cells.

关 键 词：胰腺肿瘤 干细胞 肿瘤细胞系 抗药性 肿瘤 标志物 

分 类 号：R735.9[医药卫生—肿瘤] R364.7[医药卫生—临床医学]