山东汉族人群15-脂氧合酶-1基因多态性与冠心病易感性的相关性研究  

Association of an ALOX15's polymorphism with susceptibility to coronary heart disease in the Chinese Han population from Shandong Province

作　　者：张凯[1] 黎莉[1] 刘奇迹[2] 刘芳芳[1] 马志勇[1] 龚瑶琴[2] 

机构地区：[1]教育部和卫生部心血管重构和功能研究重点实验室,山东大学齐鲁医院心内科,济南250012 [2]山东大学实验畸形学教育部重点实验室,山东大学医学院医学遗传学研究所,济南250012

出　　处：《山东大学学报（医学版）》2009年第10期102-105,109,共5页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助课题(Y2005C32);973计划资助课题(2007CB512001)

摘　　要：目的探讨山东汉族人群中15-脂氧合酶-1基因(ALOX15)多态位点rs916055T>C与冠心病(CHD)的相关性。方法应用聚合酶链式反应-限制性片段长度多态(PCR-RFLP)方法检测452例CHD患者和507例正常对照者rs916055T>C的基因型,探讨其与CHD的相关性。结果rs916055T>C基因型频率符合Hardy-Weinberg平衡定律。rs916055T>C的基因型以及等位基因频率在冠心病组和对照组间无统计学差异(P=0.398,P=0.183);而应用logistic回归分析调整了其他相关因素后,该位点基因型分布在两组间有统计学差异,等位基因C携带者(CC+CT)患冠心病的风险较TT纯合子增加了2.480倍(OR=3.480,95%CI:1.143-10.592,P=0.028)。根据冠状动脉造影结果对CHD患者进行分层分析发现,rs916055T>C与CHD严重程度无相关性。结论ALOX15的多态位点rs916055T>C可能与山东汉族人群CHD的易感性相关。Objective To explore the relationship between an ALOX15＇s polymorphism,rs916055 T〉C,and coronary heart disease（CHD）in the Chinese Han population from Shandong Province.Methods A total of 452 unrelated CHD patients and 507 unrelated control subjects were recruited in a case-control study.The genotypes of rs916055 T〉C were performed by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）.Results No significant deviation from the Hardy-Weinberg equilibrium was found in the polymorphism in the CHD or control groups.There was no significant difference in frequencies of allele and genotype in rs916055 T〉C between the CHD and control groups.After adjusting for other potential confounders including age,gender,BMI,SBP,DBP,Glucose,TG,T〉C,HDL-C and LDL-C,there was a significant difference in the genotype frequencies of rs916055 T〉C between the two groups,and the carriers of the C allele（CC＋CT）of rs916055 T〉C had a significantly higher susceptibility to CHD than the TT homozygotes（OR=3.480,95% CI：1.143-10.592,P=0.028）.No association was found between the severity of CHD and rs916055 T〉C.Conclusion rs916055 T〉C of ALOX15 is associated with the susceptibility of CHD in this Chinese Han population from Shandong Province.

关 键 词：花生四烯酸盐15-脂氧合酶 冠状动脉疾病 多态性 单核苷酸 

分 类 号：R541.4[医药卫生—心血管疾病]