银杏内酯及银杏黄酮对脑淋巴阻滞后鼠脑实质微循环血流量变化的影响  

Changes of Cerebral Blood Flow Following Subarachnoid Hemorrhage After Blockade of Cerebral Lymphatic Drainage and the Influence of Ginkgolide and Ginkgetin

作　　者：程子翠[1] 孙保亮[2] 杨明峰[2] 袁慧[2] 张颜波[2] 

机构地区：[1]泰安市中医医院脑病康复中心,山东泰安271000 [2]山东省高校脑微循环重点实验室泰山医学院附属医院神经内科

出　　处：《中国微循环》2009年第5期355-358,共4页Journal of Chinese Microcirculation

基　　金：国家自然科学基金资助项目(No.30570651;No.30670724;No.30770759);山东省教育厅科技计划项目(J05L10);山东省中医药科技发展计划项目(2005-231);山东省卫生系统高层次人才基金;泰安市大学生科技创新行动计划项目(2007D1041)资助

摘　　要：目的探讨蛛网膜下腔出血(SAH)后脑淋巴引流阻滞对脑缺血的影响和银杏内酯、银杏黄酮的保护作用。方法将雄性Wistar大鼠64只分为对照组、SAH组、SAH+脑淋巴引流阻滞(CLB)组、SAH+CLB+溶剂组、SAH+CLB+银杏内酯组(又分20mg、80mg/kg组)、SAH+CLB+银杏黄酮组(又分50mg、200mg/kg组)。于第二次SAH后3d,用激光多普勒血流计针式探头记录脑实质局部血流量(rCBF);放免法测定血浆内皮素-1(ET-1)含量。结果第二次SAH后3d,可见脑实质血流量明显降低,尤以SAH+CLB组、SAH+CLB+溶剂组降低较为显著。SAH后血浆ET-1含量增加,SAH+CLB组、SAH+CLB+溶剂组ET-1含量增加更为明显。银杏内酯、银杏黄酮可减轻CLB对SAH所致脑实质微循环血流量降低,降低血浆ET-1含量,且呈剂量依赖性。结论脑淋巴引流阻滞可显著加重SAH后脑实质血流量下降,银杏内酯和银杏黄酮对之具有一定改善作用。Objective To investigate the effects of blockade of cerebral lymphatic drainage on cerebral blood flow following subarachnoid hemorrhage and the protective effects of ginkgolide and ginkgetin. Methods The rats were divided into normal control, SAH group, SAH ＋ CLB group, SAH ＋ CLB ＋ Solvent group, higher and lower doses of ginkgolide （20mg, 80mg/kg） groups, higher and lower doses of ginkgetin （50mg, 200mg/kg） groups. On the third day after SAH, the regional cerebral essence blood flow （rCBF） was recorded with the laser Doppler. Plasam ET-1 was detected by radio immunoassay. Results The rCBF obviously reduced on the third day after SAH. Plasam ET-1 increased significiantly after SAH. The above pathological al- ternations were more severe in SAH ＋ CLB group and SAH ＋ CLB ＋ Solvent group than those in SAH group. Ginkgolide and ginkgetin partly attenuated above pathological alternations. Conclusion Cerebral lymphatic drainage pathway might play an important role in the pathophysiolagy of cerebral ischemic injury after SAH. Ginkgolide and ginkgetin may relieve the deterioration of the decreased cerebral blood flow by CLB after SAH.

关 键 词：脑淋巴引流 蛛网膜下腔出血 脑血流 银杏内酯 银杏黄酮 

分 类 号：R743.35[医药卫生—神经病学与精神病学]