心房颤动心房纤维化及血小板源生长因子受体β亚型表达的研究  

Study of the atrial fibrosis and the expression of PDGF receptor-β during atrial fibrillation

作　　者：崔明玉[1] 郭琳娜[1] 郭继鸿[2] 刘元生[2] 

机构地区：[1]内蒙古医学院组织胚胎学教研室,内蒙古呼和浩特010059 [2]北京大学人民医院,北京100044

出　　处：《中国现代医学杂志》2009年第19期2903-2906,共4页China Journal of Modern Medicine

摘　　要：目的探讨心房颤动(房颤)心房纤维化发生的相关机制。方法杂种犬15条,随机分3组:正常对照组、单纯房颤组和房颤+Miberfradil组。采用VanGieson染色、免疫组织化学方法,结合高清晰彩色图像分析系统,观察正常对照组、单纯房颤组和房颤+Miberfradil组心房组织总胶原、Ⅲ型胶原及血小板源生长因子受体β亚型(PDGFR-β)的表达。结果①单纯房颤组和房颤+Miberfradil组心房组织总胶原均明显高于正常对照组(P<0.05)。房颤+Miberfradil组的总胶原表达低于单纯房颤组,差异有显著性(P<0.05)。②单纯房颤组Ⅲ型胶原的表达明显高于正常对照组(P<0.05)。房颤+Miberfradil组Ⅲ型胶原的表达低于单纯房颤组,差异有显著性(P<0.05)。③单纯房颤组与正常对照组相比PDGFR-β蛋白的表达下降(P<0.05),房颤+Miberfradil组与单纯房颤组相比,PDGFR-β蛋白的表达明显下降(P<0.05)。结论①持续性房颤心房组织有明显的心房纤维化改变,T型钙通道阻滞剂能够减轻心房纤维化的程度。②持续性心房颤动6个月犬心房组织PDGFR-β蛋白的表达减少。[ Objective ] To investigate the possible mechanisms of atrial fibrosis during atrial fibrillation. [ Methods] Fifteen adult mongrel dogs were randomly divided into 3 groups： control group, pure atrial fibrillation group and atrial fibrillation ＋ mibefradil group. Van-Gieson staining, immunohistochemical method combined with High- resolution color image analysis system were used to detect the expressions total cardiac collagen, cardiac collagen subtype Ⅲ and PDGF receptor-β subunit in the three groups. [Results] The total cardiac collagen was significantly higher in pure atrial fibrillation group and atrial fibrillation ＋ mibefradil group when compared with that of control group （P 〈0.05）. The expression of total cardiac collagen in atrial fibrillation ＋ mibefradil group was significantly lower than that of pure atrial fibrillation group （P 〈0.05）. The expression of cardiac Collagen subtype Ⅲ was significantly higher in pure atrial fibrillation group than that of control group （P 〈0.05）. The expression of cardiac collagen subtype m in atrial fibrillation ＋ mibefradil group was significantly lower than that of pure atrial fibrillation group （P 〈0.05）. The expression of PDGF receptor-β subunit in atrial fibrillation group was decreased than that of control group （P 〈0.05）. The expression of receptor-β subunit in atrial fibrillation ＋ mibefradil group was greatly decreased than that of atrial fibrillation group （P 〈0.05）. [ Conclusion ] Atrial fibrosis was observed in atrial tissues during persistent atrial fibrillation, which could be relieved by T-type Ca^2＋ channel blocker. The expression of PDGF receptor- β subunit was decreased with persistent atrial fibrillation lasting for 6 months.

关 键 词：心房颤动 胶原 心房纤维化 血小板源生长因子 

分 类 号：R-332[医药卫生]