侧脑室注射链脲酶素导致大鼠脑内能量代谢障碍和视空间记忆障碍  

Intracerebroventricular Injection of Streptozotocin Caused Brain Energy Deficiency and Spatial Memory Impairment in Rats

作　　者：褚文政[1] 刘宏[1] 于国平[1] 孙永安[2] 钱采韻[3] 

机构地区：[1]青岛大学医学院附属烟台毓璜顶医院神经内科,山东烟台264000 [2]北京大学第一医院神经内科,北京100034 [3]中山大学第一附属医院神经内科,广东广州510080

出　　处：《中国神经免疫学和神经病学杂志》2009年第6期418-420,437,共4页Chinese Journal of Neuroimmunology and Neurology

基　　金：山东省烟台市科学技术发展计划基金资助项目(2009155)

摘　　要：目的探讨脑内胰岛素/胰岛素受体信号系统功能紊乱与认知障碍的关系。方法采用2次双侧侧脑室注射链脲酶素(streptozotocin,STZ)方法建立大鼠模型。建模后21 d检测大鼠线粒体呼吸链酶复合物和ATP酶活性;利用高效液相法检测大鼠海马ATP、ADP和AMP水平;Morris水迷宫检测视空间记忆障碍。结果STZ组大鼠脑内琥珀酸脱氢酶(succinate dehydrogenase,SDH)、细胞色素C氧化酶(cytochrome-oxydase,CCO)、Na^+-K^+-ATPase、Ca^(2+)-Mg^(2+)-ATPase活性以及ATP和ADP水平与生理盐水对照组相比差异有统计学意义(P<0.05)。Morris水迷宫实验中,STZ组大鼠平均潜伏期、平均路径长度、撤台后2 min内经过平台次数与生理盐水对照组相比差异均有统计学意义(P<0.05)。结论脑内胰岛素/胰岛素受体信号系统功能紊乱可导致与散发性老年性痴呆相似的脑内能量代谢障碍和视空间记忆障碍。Objective To testify whether dysfunction of insulin/insulin receptor transduction system may cause brain energy deficiency and spatial memory impairment in rats. Methods Rats randomized into groups to receive intracerebroventricular （icy） injection of streptozotocin （STZ） or normal saline （NS） bilaterally at 1st and 3rd days. After 21 days of the operation, enzyme activities of succinate dehydrogenase, cytochrome-oxydase, Na^＋- K^＋-ATPase, and Ca^2＋-Mg^2＋-ATPase were measured. High performance liquid chromatography （HPLC） method was used to determine ATP, ADP and AMP concentrations. Morris water maze was used to assay spatial memory impairment. Results Rats received icv injection of STZ bilaterally at 1st and 3rd days showed decrease activities of succinate dehydrogenase, cytochrome-oxydase, Na^＋-K^＋-ATPase, Ca^2＋-Mg^2＋-ATPase and decreased ATP concentration with spatial memory impairment. Conclusions Introcerebroventricular injection of STZ caused brain ATP deficiency with spatial memory impairment, suggested dysfunction of insulin/insulin receptor transduetion system may play an important role in the pathogenesis of sporadic Alzheimer disease.

关 键 词：链脲酶素 侧脑室注射 能量 阿尔茨海默病 

分 类 号：R743[医药卫生—神经病学与精神病学]