楸树组培与快繁技术初探  

Preliminary Study on Tissue Culture and Fast Propagation Technology of Catalpa bungei

作　　者：周蓉[1] 谢焕松[1] 刘鑫燕[1] 曹云英[1] 

机构地区：[1]南通大学生命科学学院,江苏南通226007

出　　处：《安徽农业科学》2009年第32期15715-15716,15794,共3页Journal of Anhui Agricultural Sciences

基　　金：南通大学校风课题(06Z108);南通大学教学研究课题资助

摘　　要：[目的]实现楸树离体的快速繁殖,并为楸树的大规模种苗生产提供理论依据。[方法]以楸树腋芽为外植体,通过选择不同培养基和激素配比,研究楸树组培及快繁技术。[结果]筛选出效果较好的培养基,分别为腋芽萌发:N6+6-BA2.0mg/L+NAA0.005mg/L;愈伤组织诱导:MS+6-BA1.5mg/L+NAA2.0mg/L;芽分化:MS+6-BA0.2～0.5mg/L+NAA0.5～1.0mg/L;继代增殖:N6+6-BA1.0～1.5mg/L+NAA0.005～0.01mg/L;生根培养:1/2MS+NAA0.5～1.0mg/L+活性碳1g/L。[结论]用组织培养法快速繁殖楸树,能大大提高楸树的繁育速度。[ Objective] The aim was to achieve rapid propagation of Catalpa bungei and provide a theoretical basis for large-scale seedling production. [ Method ] Axillary bud of Catalpa bungei were used as explants, while different medium and hormone combinations were selected to set up the tissue culture and rapid propagation system of Catalpa bungei. [ Result] N6 ＋2.0 mg/L 6-BA ＋0. 005 mg/L NAA is an appropriate culture medium for germination of axillary bud, MS ＋ 1.5 mg/L 6-BA ＋ 2.0 mg/L NAA is the best medium for callus induction, MS ＋ 0.5 mg/L 6-BA ＋ 0.5 mg/L NAA is suitable for bud differentiatation and N6 ＋ 1.5 mg/L 6-BA ＋ 0. 005 mg/L NAA for subculture multiplication. 1/2 MS ＋0.5 mg/L NAA was of advantage to rooting culture. [ Conclusion] The propagation speed of Catalpa bungei was greatly improved by tissue culture method.

关 键 词：楸树 组织培养 腋芽 外植体 愈伤组织 

分 类 号：S722.37[农业科学—林木遗传育种]