CXCR4阳性Lewis肺癌细胞具有促发新生血管形成的特征  

CXCR4 positive cells from Lewis lung carcinoma cell line have proangiogenesic potentials

作　　者：辇伟奇[1] 陈芳琳[1] 敖绪军 陈正堂[1] 

机构地区：[1]第三军医大学新桥医院全军肿瘤研究所,重庆400037 [2]解放军第五三二医院普通外科,安徽黄山245041

出　　处：《重庆医学》2009年第22期2825-2826,2830,I0003,共4页Chongqing medicine

基　　金：国家高技术研究发展计划"863"项目(2007AA02Z129);国家自然科学基金项目(30901790);重庆市自然科学基金项目(2008BB5117)

摘　　要：目的研究CXCR4阳性Lewis肺癌细胞(LLC)在新生血管形成时的始动作用。方法激光共聚焦检测LLC细胞系中CXCR4抗原表达情况,观察小鼠移植瘤组织内CXCR4阳性LLC与新生血管的毗邻关系;以CXCR4作为磁珠分选细胞的表面标志,检测CXCR4阳性和阴性LLC中VEGF、MMP9 mRNA表达情况,同时免疫组化检测两亚群细胞移植瘤中VEGF、MMP9以及CD31表达情况。结果CXCR4阳性LLC多毗邻内皮细胞,周围形成血管样结构;CXCR4阳性LLC的VEGF mR-NA表达(0.439 8±0.059)明显高于CXCR4阴性LLC(0.289 9±0.003 1)(P<0.01);CXCR4阳性LLC的MMP9 mRNA表达(0.622 3±0.013 6)明显高于CXCR4阴性LLC(0.579 2±0.017 4)(P<0.05);CXCR4阳性LLC移植瘤组织MVD(56.87±4.83)明显高于CXCR4阴性LLC移植瘤(43.52±4.91)(P<0.01)。结论LLC中的CXCR4阳性亚群具有更强上调VEGF、MMP9表达的能力,具有促发新生血管形成的特征。Objective To study the important role of CXCR4-positive LLC in the primary stage of tumor angiogenesis. Methods Location relationship of CXCR4-positive LLC and vascular endothelial cells was observed By laser scanning confocalmicroscope （LSCM）. Isolated by magnetic cell sorting,VEGF and MMP9 mRNA were evaluated by RT-PCR in CXCR4-positive and CXCR4- negative LLC; VEGF, MMP9 and CD31 protein were analyzed by immune- histochemistry in CXCR4-positive and CXCR4-negative subsets growing tumor tissue. Results Most of CXCR4-positive LLC were close to vascular endothelial cells,aberrant vaseulature around it was forming. The expression of VEGF mRNA in CXCR4-positive LLC（0. 439810. 059） was higher than that in CXCR4- negative LLC （0. 2899±0. 0031）（P〈0.01）, the expression of MMP9 mRNA in CXCR4-positive LLC（0. 6223 ±0. 0136） was higher than that in CXCR4-negative LLC （0. 5792±0. 0174）（P〈0.05）, MVD of CXCR4-positive subsets growing（56.87±4.83） was higher than of CXCR4-negative subsets growing tumor tissue（43.52±4. 91） （P〈0.01）. Conclusion CXCR4 positive cells from Lewis lung carcinoma cell line have proangiogenesic potentials.

关 键 词：LEWIS肺癌细胞 CXCR4 新生血管形成 

分 类 号：R734.2[医药卫生—肿瘤]