松嫩草原罗布麻各器官总黄酮和槲皮素的含量测定及药材抗氧化活性评价  

Determination of Total Flavonoids and Quercetrin in Different Parts of Apocynum venetum from Songnen Grassland & Its Antioxidant Activity

作　　者：李庆华[1] 魏春雁[2] 黄九林[3] 李建东[3] 

机构地区：[1]吉林医药学院,吉林市132013 [2]吉林省农业科学院,长春市130033 [3]东北师范大学植被生态科学教育部重点实验室,长春市130024

出　　处：《中国药房》2009年第33期2592-2594,共3页China Pharmacy

基　　金：国家自然科学基金资助项目(30470180)

摘　　要：目的:测定松嫩草原罗布麻各器官总黄酮和槲皮素含量并评价其体外抗氧化活性,为有效利用罗布麻资源提供参考。方法:以超声波辅助提取罗布麻中总黄酮,用比色法和高效液相色谱法分别测定罗布麻中总黄酮和槲皮素的含量,DPPH.和.OH清除法评价其抗氧化活性。结果:松嫩草原罗布麻花和叶中总黄酮、槲皮素含量较高,其次为茎;叶和花中总黄酮提取液抗氧化活性较强,茎次之。结论:松嫩草原罗布麻叶、花和茎均可以作为天然抗氧化剂原料。OBJECTIVE： To determine the contents of total flavonoids and quereetin in different parts of Apocynum venetum collected in Songnen grassland and study their antioxidant activities so as to provide references for efficient utilization of the herbal plant. METHODS： Ultrasonic extraction was applied to extract total flavonoids. The content of total flavonoids was determined by chromatometry and the content of quercetin was determined by HPLC. The antioxidant activities of the extracts from different parts of A.venetum were evaluated by determining their abilities in scavenging 1, 1- diphenyl- 2 picrylhydrazyl （DPPH · ） radicals and· OH radicals. RESULTS： The contents of quercetin and total flavonoids were richer in both leaves and flowers of A. venetum than in stems. In addition, the total flavonoids extracted from the leaves and flowers of A.uenetum showed higher antioxidant activity than from the stems. CONCLUSION： The leaves, flowers and stems of A.venetum can all be used as raw materials of natural antioxidants.

关 键 词：罗布麻 松嫩草原 总黄酮 槲皮素 含量 抗氧化活性 

分 类 号：R284.1[医药卫生—中药学] R927[医药卫生—中医学]