炎症性肠病患者血浆TRAF1的表达及其临床意义  

Plasma levels of TRAF1 in patients with inflammatory bowel disease and its clinical significance

作　　者：沈骏[1] 冉志华[1] 聂芳[1] 童锦禄[1] 朱明明[1] 蔡青[1] 黄美兰[1] 王天蓉[1] 

机构地区：[1]上海交通大学医学院附属仁济医院消化内科上海市消化疾病研究所,上海200001

出　　处：《胃肠病学和肝病学杂志》2009年第10期896-899,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：上海市科委重点实验室项目(06DZ22027)

摘　　要：目的比较炎症性肠病患者血浆肿瘤坏死因子受体相关因子-1(tumor necrosis factor receptor-assoc iated factor-1,TRAF-1)水平和正常对照者的差异,分析血浆TRAF1水平对炎症性肠病的诊断价值以及与内镜下疾病活动性的相关性。方法共纳入62例克罗恩病患者、64例溃疡性结肠炎患者和56例正常对照者。应用酶联免疫吸附试验(Enzym e-linked immuno-sorbent assay,ELISA)分析炎症性肠病患者和正常对照者血浆中TRAF1蛋白的表达,受试者工作特征曲线(rece iver-operating characteristic,ROC)分析血浆TRAF1水平对克罗恩病和溃疡性结肠炎的诊断价值,应用Pearson相关分析研究血浆TRAF1水平与内镜下疾病活动性的相关性。结果克罗恩病患者(P=0.000)和溃疡性结肠炎患者(P=0.000)血浆TRAF1水平显著高于正常对照者,同时TRAF1对区分克罗恩病患者和正常对照者(P=0.000)以及区分溃疡性结肠炎患者和正常对照者(P=0.000)具有显著的诊断价值。克罗恩病患者血浆TRAF1表达水平和内镜下疾病活动指数呈较低的负相关(r=-0.260,P=0.041),而溃疡性结肠炎患者血浆TRAF1水平与内镜下疾病活动程度无显著相关性(r=0.029,P=0.821)。结论炎症性肠病患者血浆TRAF1水平增高,血浆TRAF1水平对区分炎症性肠病患者和正常对照者具有诊断价值,但血浆中TRAF1的水平不能反应内镜下疾病活动程度。Objective To compare plasma tumor necrosis factor receptor-associated factor-1（TRAF1） levels in patients with inflammatory bowel disease（IBD） and healthy controls,and to determine the diagnostic value of plasma TRAF1 in IBD and its correlation with endoscopic disease activity.Methods A total of 62 patients with Crohn＇s disease（CD）,64 patients with ulcerative colitis（UC） and 56 healthy controls were enrolled.Enzyme-linked Immuno-sorbent assay（ELISA） was applied to evaluate plasma TRAF1 levels in patients with IBD and in healthy controls.Receiver-operating characteristic（ROC） curves were used to determine the diagnostic value of plasma TRAF1 in CD and UC.Pearson correlation coefficient was used to analyze the correlation between plasma TRAF1 and endoscopic disease activity.Results Plasma levels of TRAF1 were significantly higher in CD（P=0.000） and UC（P=0.000） than that in healthy controls.Moreover,TRAF1 showed significant diagnostic value in distinguishing patients with CD,UC and healthy controls（P=0.000）.However,plasma TRAF1 showed a very low correlation with endoscopic disease activity in patients with CD（r=-0.260,P=0.041）,and no significant correlation was indicated between plasma TRAF1 and endoscopic disease activity in patients with UC（r=0.029,P=0.821）.Conclusion Plasma levels of TRAF1 increased in patients with IBD.TRAF1 showed diagnostic value in distinguishing patients with IBD and healthy controls.However,plasma levels of TRAF1 could not reflect endoscopic disease activity.

关 键 词：TRAF1 克罗恩病 溃疡性结肠炎 内镜下疾病活动程度 

分 类 号：R574.62[医药卫生—消化系统]