人脑胶质瘤中miR-221和p27的表达及临床意义  

Expression and significance of miR-221 and p27 in human gliomas

作　　者：赵鹏[1] 陆小明[1] 傅震[1] 鲁艾林[1] 陈云祥[1] 刘宁[1] 尤永平[1] 

机构地区：[1]南京医科大学附属第一医院神经外科,210029

出　　处：《江苏医药》2009年第11期1249-1251,共3页Jiangsu Medical Journal

基　　金：国家自然科学基金项目(30672165;30872657);江苏省自然科学基金(2008475)

摘　　要：目的探讨miR-221在胶质瘤中的表达及其与p27的关系。方法收集不同病理级别胶质瘤标本,以荧光定量PCR法和免疫印迹法检测其中miR-221及p27的表达,分析其与胶质瘤病理级别的关系。结果miR-221的表达强度与肿瘤恶性程度呈正相关,与p27的表达呈负相关。结论miR-221的过表达和p27的表达下降或缺失与胶质瘤,尤其是恶性胶质瘤的发生、发展密切相关。Objective To analyze the expression and clinical significance of miR-221 and p27 in human glioma samples. Methods The expressions of miR-221 and p27 were detected by fluorescence quantitative-PCR and Western blot in 29 glioma samples of different malignancy grades. The relationship of the expressions with the malignancy grades of the tumor was analyzed. Results The expression level of miR-221 was positively correlated with glioma malignancy and negatively correlated with the expression of p27. Conclusion The overexpression of miR-221 and downexpression is closely correlated to development and progress of human glioma.

关 键 词：MIR-221 胶质瘤 P27 

分 类 号：R735[医药卫生—肿瘤]