中国HIV／AIDS患者保守表位的2F5抗体与疾病进展关系  

Levels of antibodies 2F5 directed against conserved epitopes in gp41 association with progression in HIV-1-infected individuals in China

作　　者：张晓丽[1,2] 韩晓旭[2] 代娣[2] 王哲[3] 包名家[2] 张子宁[2] 徐东兵[2] 耿文清[2] 尚红[2] 

机构地区：[1]中国医科大学附属第一医院卫生部艾滋病免疫学重点实验室,沈阳110001 [2]佳木斯大学临床医学院检验系,154001 [3]河南省疾病预防控制中心

出　　处：《中华微生物学和免疫学杂志》2009年第10期930-933,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家科技重大专项课题资助项目（2008ZX10001001）;国家重点基础研究发展计划（973计划）（2006CB504206）;国家自然科学基金（30800969）

摘　　要：目的研究中国HIV/AIDS患者gp41保守表位中和抗体2F5与疾病进程的关系。方法应用合成4（ELDKWA）-C24肽及gp4l抗原肽包被96孔酶标板，ELISA方法检测特异性抗体水平。提取正常人外周血单个核细胞（PBMC），应用HIV-1 SF33毒株进行病毒多轮感染PBMC中和试验。结果缓慢进展者（slow progressor，SP）的2F5样中和抗体水平显著高于典型进展者（typical progressor，TP）（P〈0．05）；2F5样中和抗体水平与gp41特异性抗体水平正相关（r=0．406，P〈0．05）。结论中国HIV-1缓慢进展者体内具有高水平的2F5样抗体，2F5抗体可能是疾病不进展的保护性因素之一。Objective To study the levels of 2F5-like antibody recognized gp41 conserved epitope of HIV-1-infected individuals in China, explore the influence of 2F5-like antibody on disease progression of HIV-1 infected individuals. Methods Direct ELISA was developed for the specific comparison of levels of antibodies against gp41 and 4 （ELDKWA）-C peptides sera from HIV-1-infected individuals. Peripheral blood mononuclear cells（PBMC） were isolated from HIV-seronegative donors. Multi-round PBMC neutralization assays were performed with HIV-1 isolate SF33. The supernatant p24-Ag of HIV was analyzed. Results Levels of 2F5-1ike antibodies in the slow progressor （SP） plasma were significantly higher than those in typical progressor （TP）（ P 〈 0.05 ）. There was significant correlation with the levels of 2F5 neutralizing antibody and gp41 antibodies（r =0.406,P〈0.05）. Conclusion The levels of 2F5-1ike antibodies may influence on disease progression of HIV-infected individuals. The high levels of neutralizing antibodies may arrest disease from progressing.

关 键 词：HIV 中和抗体 GP41 2F5 

分 类 号：R512.91[医药卫生—内科学]