中国甘肃地区汉族IL-10基因多态性与胃癌关系  

Association of polymorphism of IL-10 and Helicobacter pylori infection to gastric cancer

作　　者：祝凯华[1] 李玉民[2] 刘玲玲[3] 张丹[2] 唐小凡[2] 何文婷[4] 陈应泰[2] 赵军[2] 

机构地区：[1]沭阳县人民医院普外科,江苏沭阳223600 [2]兰州大学第二医院,甘肃兰州73000 [3]兰州大学基础医学院生物化学和分子生物学研究所,甘肃兰州73000 [4]兰州大学第一医院肿瘤防治中心,甘肃兰州73000

出　　处：《第四军医大学学报》2009年第21期2411-2414,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30870364)

摘　　要：目的:探讨白细胞介素10(IL-10)启动子区-592位点的单核苷酸多态性(SNP)、及幽门螺旋杆菌(Hp)感染与甘肃地区胃癌之间易感性的关系.方法:采用聚合酶链反应-限制性片段长度多态(PCR-RFLP)技术分别对来自甘肃地区人群137例胃癌患者及与其配比的144例对照个体IL-10-592A/C基因多态性进行检测;采用免疫印迹(Immunoblot-ting)技术对幽门螺旋杆菌进行检测.结果:①对照人群IL-10基因-592位点AA,AC,CC3种基因型分布频率分别为29.9%,47.9%,22.2%;②胃癌组IL-10CC基因型携带频率为32.1%显著高于正常对照组22.2%(P<0.05),携带IL-10CC基因型个体胃癌的发病风险增高到2.19倍;③以IL-10AA基因型并血清幽门螺杆菌免疫印迹阴性的个体为对照,CC基因型并HP感染阳性及CC基因型并HpCagA阳性患病风险显著增高,分别为对照组的3.75倍,4.51倍.结论:IL-10基因-592位点A/C多态性与胃癌的遗传易感性相关;IL-10A/C基因多态性和Hp感染在胃癌发生发展过程存在加乘交互作用.AIM：To analyze the relationship both -592 site A/C polymorphism in interleukin-10（IL-10） gene and different virulence factors of Helicobacter pylori in a population from Gansu Province and susceptibility to gastric cancer. METHODS：Polymerase chain restriction fragment length polymorphism （PCR-RFLP） were performed to analyze the genotype of the A/C polymorphism in its -592 site of promoter region,and Immunoblotting was performed to test H.pylori. RESULTS：①The distribution frequency of AA,AC,CC three genotypes were 29.9% ,47.9% ,22.2% respectively in normal control. ②The frequency of CC genotype was conspicuous higher in the gastric cancer （GC） group compared to the control group （P 〈0. 05）, moreover, individuals with the IL-10AC ＋ CC genotype increased 2.19 fold risk for gastric cancer. ③Take IL-10 the AA genotype and the H. pylori-immunoblotting negative individual as the comparison, there was an obviously increased risk of developing gastric cancer for those who carried both CC genotype and H. pylori seropositive, CagA positive , increased 3. 75, 4. 51 fold risk respectively. CONCLUSION： Results indicated that there was a relationship between IL-10-592 A/C polymorphism and susceptibility to gastric cancer. And there were statistically interaction between the -592 site A/C polymorphism in IL-10 and H. pylori infection in the occurrence and development process of gastric cancer.

关 键 词：基因多态性 白介素10 胃肿瘤 幽门螺杆菌 免疫印迹 CAGA 

分 类 号：R735.2[医药卫生—肿瘤]