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作 者:史宏志[1] 赵永利[1] 杨兴有[2] 谢子发 刘国顺[1] 靳冬梅[2] 周开旭 张定贵[2]
机构地区:[1]河南农业大学国家烟草栽培生理生化研究基地,郑州文化路95号450002 [2]四川达州烟草科研所,达州635000
出 处:《中国烟草学报》2009年第5期28-32,共5页Acta Tabacaria Sinica
基 金:四川省烟草公司2008年科技项目"四川优质白肋烟生产理论与技术研究应用";合同号:200703005
摘 要:采用气相色谱法对具有不同烟碱转化能力白肋烟烟株不同叶位和叶点的烟碱转化率进行测定。结果表明,非转化株和高转化株不同叶位以及同一叶片不同叶点的烟碱转化率分布比较一致,变异性较小,非转化株各样品烟碱转化率均在2%以下,高转化株烟碱转化率均在85%以上,不表现烟碱转化的嵌合特征,取样部位对转化株鉴别效果无显著影响。低转化和中转化烟株,不同叶位烟叶和不同叶点间烟碱转化率存在较大的变异性,表现明显的嵌合特征,低转化株不同叶位烟叶烟碱转化率的变异范围为2.5%~4.8%,不同叶点的变异范围为2.6%~7.0%;中转化株不同叶位烟碱转化率的变异范围为2.51%~90.6%,不同叶点的变异范围为2.7%~66.9%。由于一部分叶片和叶点的烟碱转化率低于3%的现行转化株鉴别标准,取样部位会对转化株的鉴别效果产生影响,降低转化株鉴别标准十分必要。GC method was used to analyze the nicotine conversion rate of burley samples from different leaf positions and leaf areas of plants with different conversion levels.The results showed that for non-converters and high converters,the distribution of nicotine conversion rate among different leaf positions and leaf areas was consistent with small variation;nicotine conversion rates for all the samples were below 2% for non-converters and were above 85% for high converters,with no chimera characteristics;so the sampling position had no impacts on identification results.For low converters and medium converters,great variation of nicotine conversion rate existed among different leaf positions and leaf areas,and the chimera characteristics were displayed.The range of nicotine conversion rate for low converters was 2.5%~4.8% for leaf positions,and 2.6%~7.0% for leaf areas.The range of nicotine conversion rate for medium converters was 2.51%~90.6% for leaf positions,and 2.7%~66.9% for leaf areas.Since the nicotine conversion rate of certain leaf samples were below the current converter standard,the sampling position would have great impact on converter identification,so lowering the standard was quite necessary for more efficient screening.
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