miR-21和miR-125在结直肠癌中的表达及其与临床病理指标的关系  

Clinicopathological significance of microRNA-21 and miR-125 expression in colorectal cancer

作　　者：张益[1] 周总光[1] 王玲[1] 张鹏[1] 王默进[1] 崔长富[1] 关键婷[1] 陈柯玲[1] 詹兰[1] 

机构地区：[1]四川大学华西医院生物治疗国家重点实验室消化外科研究室,成都610041

出　　处：《中华胃肠外科杂志》2009年第6期623-626,共4页Chinese Journal of Gastrointestinal Surgery

基　　金：美国纽约中华医学CMB基金

摘　　要：目的探讨miR-21和miR-125在结直肠癌中的表达与临床病理特征之间的关系。方法选取2006年7月至2007年12月经手术切除的原发性结直肠癌标本100例．采用实时荧光定量PCR分析miR-21和miR-125的表达情况。结果相对于癌旁组织．100例结直肠癌组织中miR-21表达上调2．3倍（P=0．025）。其表达与TNM分期（P=0．028）以及浸润深度有关（P=0．023）：而miR-125在癌组织中的表达则下调3．3倍（P=0．005），其表达与TNM分期、浸润深度和远处转移无明显关联（P〉0．05）。结论miR-21可能参与了结直肠癌的发生和发展：而miR-125可能未参与。Objective To investigate the expression of microRNA（miR）-21 and miR-125 in colorectal cancer （CRC） and its relationship with clinicopathological features. Methods Quantitative real-time PCR was applied to examine the expression of miR-21 and miR-125 in 100 primary CRC specimens which were diagnosed and operated in West China Hospital between 2006 and 2007, in comparison with the corresponding normal mucosa specimens. The relationship between the expression of miRNAs and clinicopathological features was analyzed. Results The expression of miR-21 in CRC was up-regulated by 2.3 times compared to normal mucosa （P=0.025）, while the expression of miR-125 was down-regulated by 3.3 times in comparison with normal mucosa （P=0.O05）. Furthermore, the expression of miR-21 was related to TNM stage （P=0.028） and local invasion （P=0.023）. On the other hand, no significant relationship was found between the expression of miR-125 and clinicopathological features （P〉0.05）. Conclusion The over-expression of miR-21 may play a role in the development and progression of CRC, while miR-125 may not be related to the pathogenesis of CRC.

关 键 词：结直肠肿瘤 实时荧光定量聚合酶链反应 微RNAS 

分 类 号：R735[医药卫生—肿瘤]