Caveolin-1蛋白及ERK1/2信号转导途径在17β-雌二醇抑制血管平滑肌细胞增殖中的作用  

Effect of caveolin-1 and ERK1/2 on 17β-estradiol-induced inhibition of VSMC proliferation

作　　者：刘海梅[1] 林桂平[2] 徐进文[2] 王庭槐[2] 

机构地区：[1]广州中医药大学生理教研室,广东广州510006 [2]中山大学中山医学院生理教研室,广东广州510080

出　　处：《中国病理生理杂志》2009年第11期2093-2098,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30440028)

摘　　要：目的:研究caveolin-1蛋白及细胞外信号调节激酶(ERK1/2)在17β-雌二醇(E2)抑制血管平滑肌细胞(VSMCs)增殖中的作用。方法:在培养的VSMCs上,采用[3H]-TdR掺入法、Western blotting观察E2预处理前后血清(FCS)对VSMCs DNA合成及caveolin-1、MKP-1、磷酸化ERK1/2蛋白表达的影响,同时采用RT-PCR技术观察caveolin-1 mRNA的变化。结果:E2作用24h,可明显抑制FCS诱导的VSMCs增殖。RT-PCR及Western blotting结果显示,FCS可抑制caveolin-1 mRNA及蛋白表达,而E2预处理可增加其表达。同时,Western blotting结果还提示,E2预处理可增加MKP-1蛋白表达,抑制ERK1/2蛋白表达。结论:E2可通过增加caveolin-1及MKP-1蛋白表达,抑制磷酸化ERK1/2活性,促进其降解,来抑制VSMCs增殖。AIM： To investigate the effect of eaveolin -1 and phosphorylation of ERKI/2 on 17β-estradiol （ E2 ） induced inhibition of vascular smooth muscle cells （VSMCs）. METHODS： The proliferation in cultured VSMCs was determined by using [3H ] - thymidine incorporation. The expressions of caveolin - 1, MKP - 1 and ERK1/2 phosphorylation were measured by Western blotting. The expression of caveolin - I mRNA was measured by RT - PCR. RESULTS： Exposed to fetal calf serum （FCS） for 24 h, the increase in proliferation of VSMCs was detected by [ 3H ] -thymidine incorporation. Pretreatment with various concentrations of E2 for 24 h inhibited VSMC proliferation induced by FCS. The results of Western blotting and RT - PCR showed that pretreated with 17β- estradiol for 24 h reserved the decrease in caveolin - 1 induced by FCS. Western blotting results further proved that the expression of MKP - 1 was significantly increased and the expression of ERK1/2 phosphorylation was decreased after incubated with 17β - estradiol. CONCLUSION： 17β- estradiol increases caveolin - 1 and MKP - 1 expressions, and decreases ERK1/2 phosphorylation, leading to the inhibition of VSMC proliferation.

关 键 词：雌二醇 血管平滑肌细胞 细胞质膜微囊蛋白1 细胞外信号调节激酶 

分 类 号：R331.36[医药卫生—人体生理学]