难治性癫患儿多药耐药基因的表达及意义  

The expression and significance of multidrug resistance gene in children with refractory epilepsy

作　　者：庞保东[1] 董琰[1] 张润春[1] 刘寅[1] 刘树平[1] 曹丽华[1] 吴家骅[1] 

机构地区：[1]华北煤炭医学院附属唐山市妇幼保健院,河北唐山063000

出　　处：《临床儿科杂志》2009年第11期1023-1025,共3页Journal of Clinical Pediatrics

基　　金：河北省科学技术研究与发展项目(No.042761107)

摘　　要：目的研究难治性癫(RE)患儿多药耐药基因(MDR1)的表达及其临床意义。方法提取难治性癫患儿(n=30)、非难治性癫患儿(n=30)和正常健康儿童(n=30)外周血标本,用荧光定量PCR方法分析比较MDR1 mRNA在各组的表达。同时观察MDR1 mRNA水平与癫发作频率和应用抗癫药物(AEDs)的种类与数量关系。结果MDR1 mRNA在难治性癫组表达明显高于非难治性癫组及正常对照组(P均<0.01);在发作次数频繁患儿明显高于发作次数较少患儿(P<0.01);在使用4种AEDs患儿明显高于使用2种及3种AEDs患儿(P<0.05)。结论血中高表达的MDR1参与了难治性癫的耐药机制,可作为判断难治性癫的客观指标。Objective To study the expression and significance of muhidrug resistance gene （MDR1） in children with refractory epilepsy （RE）. Methods Children with RE （n = 30）, non-RE （n = 30） and healthy children （n=30） were collected. The expression of MDRl-mRNA in peripheral blood was analyzed by fluorescence quantitative PCR. The relationship of MDRI-mRNA with epileptic frequency and numbers of antiepileptic drugs （AEDs） were observed. Results The expression of MDR1 in RE group obviously increased when compared with that of non-RE group and healthy group （P 〈 0.01, P 〈 0.01 ） ; MDR1 expression was more among patients with high frequent epilepsy than patients with low frequent epilepsy （P 〈 0.01 ） ; more in patients administered with four kinds of AEDs than those with two or three kinds of AEDs （P 〈 0.01）. Conclusions MDR1 overexpression in blood of children with RE may be linked to drug-resistant mechanism of RE. It might be used as a clinical indicator of RE.

关 键 词：难治性癫 多药耐药基因 荧光定量PCR 儿童 

分 类 号：R742.1[医药卫生—神经病学与精神病学]