环孢素A对未成熟脑惊厥性损伤的保护作用及机制研究  

Study on the protective function and its mechanism of cyclosporin A to immature brain tissue with convulsive brain damage

作　　者：郭亚乐[1] 黄绍平[1] 李丹[1] 杨琳[1] 周戬平[1] 

机构地区：[1]西安交通大学医学院第二附属医院儿科,陕西西安710004

出　　处：《临床儿科杂志》2009年第11期1030-1035,共6页Journal of Clinical Pediatrics

基　　金：国家自然科学基金资助项目(No.30672263);陕西省科技攻关资助项目〔No.2006k15-G1(1)〕

摘　　要：目的探讨环孢素A(CsA)对未成熟脑惊厥性损伤的保护作用及机制。方法21日龄SD雄性大鼠67只,制作氯化锂-匹鲁卡品癫模型,分别于制模后6、30、54 h采用CsA干预,分5、10、25 mg/(kg.次),三个干预剂量,与模型不干预组对比,观察制模后72 h脑海马CA1区凋亡细胞、多药耐药基因产物P-糖蛋白(P-gp)、星形胶质细胞纤维酸性蛋白(GFAP)表达情况等。结果模型组比假手术组海马CA1区凋亡细胞、P-gp、GFAP表达明显增加,CsA 5 mg/(kg.次)干预组可显著减少P-gp、GFAP表达,且与假手术组水平接近,CsA 10 mg/(kg.次)、CsA 25 mg/(kg.次)有相似效果,但不及CsA 5 mg/(kg.次)效果明显。CsA干预不能减少凋亡细胞。结论中小剂量CsA可以通过降低海马CA1区、P-gp、GFAP表达而减轻未成熟脑惊厥性损伤。Objective To investigate the protective function and its mechanisms of cyclosporin A to immature brain tissue with convulsive brain damage. Methods 21-day-old SD rats were given lithium-pilocarpine to make the epilepsy model. Total 67 male rats had been investigated. Cyclosporin A （CsA） were injected three times at 6, 30, 54 hrs after model had been established. Three dosages had been chosen： 5, 10 and 25 mg/kg each time. The level of apoptotic cells, P-glycoprotein （P-gp）, glial fibrillary acidic protein （GFAP） in CA1 area of hippocampus had been determined, and compared with the rats without giving CsA. Results Rats from epilepsy model group had higher level of apoptosis, P-gp, GFAP expression than those from pseudo-model group. CsA injection by dose 5 mg/kg each time for three times reduced the level of P-gp, GFAP. Model group and pseudo-model group were same. Both the interventions of CsA injection by 10 mg/kg and 25 mg/kg can reduce the level of P-gp, GFAP, however neither of their effectiveness was better than CsA 5 mg/kg each time. Conclusions Small dosage of CsA may protect the immature brain tissue from convulsive brain damage by reducing the level of P-gp, GFAP in CA1 area of hippocampus.

关 键 词：环孢素A 癫 未成熟脑 星形胶质细胞纤维酸性蛋白 P-糖蛋白 

分 类 号：R742.1[医药卫生—神经病学与精神病学]