电针结合重复经颅磁刺激对脑缺血大鼠海马突触后致密物-95的影响  

Effect of electroacupuncture combined with repetitive transcranial magnetic stimulation on postsynaptic density 95 in the hippocampus of rats after cerebral ischemia

作　　者：韩肖华[1] 黄晓琳[1] 王熠钊[1] 陈红[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院康复医学科,武汉430030

出　　处：《中华物理医学与康复杂志》2009年第11期725-728,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：国家自然科学基金资助项目（30672216/C030320）,华中科技大学同济医学院附属同济医院科研基金（2008）

摘　　要：目的探讨电针结合重复经颅磁刺激对脑缺血大鼠海马突触后致密物-95（PSD-95）的影响。方法60只Wistar大鼠随机分为正常组、模型组、电针组（EA组）、重复经颅磁刺激组（rTMS组）和电针结合重复经颅磁刺激组（EA＋rTMS组），每组又根据观察时间点分为7d、14d和28d3个亚组，每个亚组4只大鼠。大鼠复制大脑中动脉栓塞模型后，分别给予电针、重复经颅磁刺激和电针结合重复经颅磁刺激干预，免疫组织化学法检测缺血侧海马齿状回和CA3区的PSD-95蛋白表达变化。结果观察第7天，模型组、EA组、rTMS组和EA＋rTMS组大鼠海马的PSD-95表达均下降；治疗14d后，各治疗组PSD-95表达上调；至28d时各治疗组与模型组比较差异有统计学意义，尤其是EA＋rTMS组PSD-95在海马CA3区表达增加更明显，与EA组和rTMS组比较差异有统计学意义。结论电针结合重复经颅磁刺激能明显增强脑缺血大鼠海马PSD-95的表达，这可能是其改善脑缺血大鼠学习记忆功能的机制之一。Objective To investigate the effect of electroacupuncture （EA） combined with repetitive transcranial magnetic stimulation （rTMS） on postsynaptic density 95 （PSD-95） in the hippocampus of rats after cerebral ischemia. Methods Sixty healthy male Wistar rats were divided into normal, model, EA, rTMS and EA ＋ rTMS groups randomly, and the rats in each group were further divided into 7 d, 14 d and 28 d subgroups. A model of middle cerebral artery occlusion （MCAO） was established, followed by EA, rTMS or EA ＋ rTMS treatment. The expression of PSD-95 in the dentate gyrus and area CA3 of the hippocampus of the ischemic cerebral hemisphere were investigated. Results The expression of PSD-95 in the dentate gyrus and area CA3 of the ischemic cerebral hemisphere had decreased 7 d after cerebral ischemia. The expression of PSD-95 in the three treatment groups increased after 14 d of treatment and was significantly greater compared with the model group after 28 d of treatment. The EA ＋ rTMS group was especially superior to the EA and rTMS groups in terms of the expression of PSD-95 in area CA3 by the 28th day. Conclusions EA ＋ rTMS can significantly increase the expression of PSD- 95 in the hippocampus after cerebral ischemia. This may help explain how EA ＋ rTMS improves learning and memory after cerebral ischemia.

关 键 词：电针 重复经颅磁刺激 脑缺血 海马 突触后致密物-95 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R245[医药卫生—临床医学]